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Journal of Lipid Research, Vol 29, 1139-1148, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Characterization of dog prenodal peripheral lymph lipoproteins. Evidence for the peripheral formation of lipoprotein-unassociated apoA- I with slow pre-beta electrophoretic mobility

M Lefevre, CH Sloop and PS Roheim
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112-2822.

Dog plasma and prenodal peripheral lymph apoA-I distribution was examined by nondenaturing gradient gel electrophoresis-immunoblot analysis. In control dogs, plasma apoA-I could be localized to two distinct populations of particles with modal diameters of 8.4 nm and 10.4 nm. The smaller sized population accounted for over 50% of plasma apoA-I. Peripheral lymph apoA-I distribution was significantly different. The percentage of apoA-I localized to the 10.4 nm population was reduced by 40% and the modal diameter of the smaller HDL apoA-I population was significantly decreased by 0.1 nm. Additionally, peripheral lymph apoA-I could be localized to particles smaller than albumin (lipoprotein-unassociated apoA-I). The presence of lipoprotein- unassociated apoA-I particles was confirmed by gel filtration chromatography. Immunoblots of column fractions subjected to agarose electrophoresis revealed that these particles had slow pre-beta electrophoretic mobility. In dogs fed an atherogenic diet, lipoprotein- unassociated apoA-I particles with slow pre-beta electrophoretic mobility could be found in both plasma and peripheral lymph. With increasing degree of hypercholesterolemia, the relative amount of plasma lipoprotein-unassociated apoA-I tended to increase. In peripheral lymph, an increasing degree of hypercholesterolemia was associated with a decrease in the relative amount of lipoprotein- unassociated apoA-I. Instead, a population of large apoA-I particles (11-25 nm) became increasingly prominent.
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