HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Smeets, H. J. Articles by Wieringa, B. Search for Related Content PubMed PubMed Citation Articles by Smeets, H. J. Articles by Wieringa, B. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 29, 1231-1237, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Identification of apolipoprotein E polymorphism by using synthetic oligonucleotides

HJ Smeets, J Poddighe, PM Stuyt, AF Stalenhoef, HH Ropers and B Wieringa
Department of Human Genetics, Radboud Hospital, University of Nijmegen, The Netherlands.

A procedure based on selective hybridization with allele-specific oligonucleotides was developed for typing apolipoprotein E variants from human genomic DNAs. Two sets of oligonucleotides were synthesized and used to discriminate either between epsilon 3 and epsilon 4 alleles or between epsilon 3 and epsilon 2 alleles. Combination of the allele- specific oligonucleotide hybridization with the method for in vitro DNA amplification (Polymerase Chain Reaction) Saiki, R. K. et al. 1985. Science, 230: 1350-1354) (1) dramatically improved the sensitivity and the reliability of the procedure. Adaptation of a simple strategy involving direct cloning and DNA sequencing of in vitro amplified DNA enables rapid identification of any mutation within the apoE gene area encoding the receptor binding domain.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?