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Journal of Lipid Research, Vol. 3, 111-116, January 1962
Copyright © 1962 by Lipid Research, Inc.

Dextran sulfate precipitation and ultracentrifugation of lipoproteins from hypercholesterolemic dog serum

Toshio Sakagami and D. B. Zilversmit

Department of Physiology, University of Tennessee, Memphis 3, Tennessee

Dogs fed a diet containing butter, cholesterol, and thiouracil develop severe hypercholesterolemia. Both ₂- and ßbeta;-lipoprotein cholesterol and phospholipid increase whereas these lipids in the ₁ fraction appear to decrease. Comparison of the dextran sulfate-precipitable lipoproteins with lipoprotein fractions obtained by ultracentrifugation at densities 1.019, 1.063, and 1.21 and with paper electrophoretograms reveals that the addition of low molecular weight dextran sulfate and calcium chloride to diluted hypercholesterolemic dog serum quantitatively precipitates low-density lipoproteins. Paper electrophoresis revealed that the dextran sulfate-precipitable fraction contains both ₂- and ßbeta;-lipoproteins.

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