Journal of Lipid Research, Vol. 3, 120-125, January 1962
Copyright © 1962 by Lipid Research, Inc.
A simple chromatographic technique for removal of non-lipid contaminants from lipid extracts
J. J. Biezenski
Department of Hematology, Laboratory Division, Montefiore Hospital, New York 67, New York
A simple procedure for the removal of non-lipid contaminants from lipid extracts is described. The lipids are applied in a line to a silicated paper and eluted in a descending manner into a beaker with 20% methanol in choloform. The impurities remain on the paper. The procedure, which is carried out in an atmosphere as anhydrous as is practical, is best conducted at 30° and takes 16 to 24 hours. The amount of lipid material that can be purified on one paper is equivalent to that extracted from 1 g of tissue. The recovery of all classes of lipids is close to 100% without selective loss or obvious degradation. Chromatographic proof is provided for the removal of all ninhydrin and ammoniacal silver nitrate-staining contaminants. Inorganic phosphate present in the lipid extract is also completely removed but other salts may persist in very small quantities. The procedure was applied to lipids extracted from rat liver, kidney, and heart. Quantitative analyses of all the nitrogen-containing residues were carried out and the sum agrees well with the total nitrogen. Chromatography of the acid hydrolysates revealed only two ninhydrin-staining compounds, identified as serine and ethanolamine.
Submitted on May 26, 1961
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