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Journal of Lipid Research, Vol. 3, 56-59, January 1962
Copyright © 1962 by Lipid Research, Inc.

Biosynthesis of linoleic acid from 1-C¹⁴-cis-2-octenoic acid by the laying hen

Raymond Reiser , N. L. Murty , and Henry Rakoff

Department of Biochemistry and Nutrition, Texas Agricultural Experiment Station, Texas A. and M. College, College Station, Texas

A white leghorn hen was given 0.2 me of 1-C¹⁴-cis-2-octenoic acid in one 1.05-g dose, and five eggs laid subsequently were collected. Linoleic acid isolated from the eggs, both as the tetrabromide and by preparative gas-liquid chromatography, was found to be radioactive. This was proven to be octadeca-9, 12-dienoic acid by characterizing the products of oxidation—caproic and azelaic acids, and 3 CO₂. The caproic acid, which was isolated by gas-liquid chromatography from periodate-permanganate oxidation of the labeled linoleic acid, was found to be inactive. The carbon 11 of the linoleic acid was isolated as BaCO₃ after oxidation with KMnO₄ in acetic acid and was found to be over 4.3 times as radioactive as the active azelaic acid carbons. It was concluded that the hen synthesized octadeca-9, 12-dienoic acid from the 1-C¹⁴-cis-2-octenoic acid.

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