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Journal of Lipid Research, Vol. 3, 184-189, April 1962
Copyright © 1962 by Lipid Research, Inc.

Phospholipids of Azotobacter agilis, Agrobacterium tumefaciens, and Escherichia coli

Tsuneo Kaneshiro and Allen G. Marr

Department of Bacteriology, University of California at Davis, Davis, California

The principal lipid of A. agilis, A. tumefaciens, and E. coli extractable with ethanol and methanol-chloroform was a phosphatidyl ethanolamine. A phosphatidyl choline was detectable only in A. tumefaciens. The fatty acids of phospholipid from A. agilis were found to be myristic (7%), palmitic (35%), palmitoleic (41%), and octadecenoic (17%) acids. The fatty acids from A. tumefaciens were myristic (1%), palmitic (15%), hexadecenoic (1%), methylene hexadecanoic (6%), octadecenoic (30%), and lactobacillic (47%) acids. The neutral lipid was a minor fraction (5% to 10%) of the extractable lipids and was found to consist of free fatty acids, esters, and coenzyme Q.

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