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Journal of Lipid Research, Vol. 3, 234-237, April 1962
Copyright © 1962 by Lipid Research, Inc.

Plasmalogens and phosphatides of rat diaphragm after incubation with palmitate-1-C¹⁴ in vitro

Martin Colodzin , Edgar M. Neptune Jr. , and Herschel C Sudduth

Naval Medical Research Institute, National Naval Medical Center, Bethesda 14, Maryland

Ethanolamine-containing plasmalogen was found to be the major type of plasmalogen in rat diaphragm. The molar ratio of total plasmalogen to total phosphatide phosphorus was 0.09. After incubation of rat diaphragm with palmitate-1-C¹⁴, it was found that the specific activity of diglyceride always exceeded that of both cephalin and lecithin, and the specific activity of the phosphatides approached that of the diglyceride with increasing time of incubation. Lecithin specific activity always exceeded that of cephalin. Estimated turn-over times were approximately 2 hours for lecithin, 3 hours for triglyceride and 10 hours for cephalin.

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