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Journal of Lipid Research, Vol. 3, 269-274, April 1962
Copyright © 1962 by Lipid Research, Inc.

A silicic acid adsorption method for the determination of ganglioside sialic acid

R. H. McCluer , E. H. Coram , and H. S. Lee

Columbus Psychiatric Institute and Hospital and Department of Physiological Chemistry, Ohio State University, Columbus 10, Ohio

A semimicro method for the analysis of ganglioside sialic acid is presented. The method is based upon the fact that brain gangliosides can be adsorbed on a small amount of silicic acid from 92% n-butanol and eluted with methanol. Recovery of added gangliosides was found to be 93.5%, and the coefficient of variation for the analysis of 5 to 50 mg of tissue was found to be ±4.4%.

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