Journal of Lipid Research, Vol. 3, 460-466, October 1962
Copyright © 1962 by Lipid Research, Inc.
The differential migration of sterol acetates on silica gels and its application to the fractionation of sterol mixtures
Peter D. Klein and Patricia A. Szczepanik
Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois
The retention volumes of 12 sterol acetates relative to cholesteryl acetate were determined on four well-characterized silica gels of different body structure. These values illustrate the contribution of silica gel structure to the chromatographic process and permit the selection of appropriate gels for a given separation. The application of these values to the fractionation of model and biological mixtures of sterol acetates is illustrated.
Submitted on June 14, 1962