| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 30, 1525-1534, Copyright © 1989 by Lipid Research, Inc.
L Lagrost, P Gambert, M Boquillon and C Lallemant
The distribution of human apolipoprotein A-IV was studied in sera from
normolipidemic fasting subjects by high performance gel filtration on a
Superose 12 HR column. The major part of apolipoprotein A-IV eluted in the
range of the apolipoprotein A-I peak, and distributed mainly in the
large-size high density lipoprotein subfractions. Only a small peak or a
shoulder on the main fraction appeared in the elution volume of free
apolipoprotein A-IV. To investigate the relation of apolipoprotein A-IV
with high density lipoprotein particles, serum high density lipoproteins
were precipitated by incubating human serum with anti- apolipoprotein A-I
immunoglobulins. At optimal concentrations, inducing a precipitation of 90
to 95% of serum apolipoprotein A-I, about 70% of serum apolipoprotein A-IV
was precipitated. It was concluded that, in fasting human serum,
apolipoprotein A-IV was mainly associated with high density lipoprotein
particles. This high degree of association to high density lipoproteins did
not result from the known in vitro redistribution of apolipoprotein A-IV
induced by lecithin: cholesterol acyltransferase activity since it was
observed in sera in the presence of inhibitors of this enzyme. The
comparison of gel filtration profiles of total serum and of serum fractions
separated by ultracentrifugation showed that the apolipoprotein A-IV-high
density lipoprotein association was a weak one, easily dissociated by the
ultracentrifugation process. The existence in fasting human serum of a
predominant high density lipoprotein-associated form of apolipoprotein A-IV
should stimulate more studies of the general function and metabolism of
this protein.
ARTICLES
Evidence for high density lipoproteins as the major apolipoprotein A-IV- containing fraction in normal human serum
Laboratoire de Biochimie Medicale, Faculte de Medecine, Dijon, France.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. R. Tubb, R. A. G. D. Silva, K. J. Pearson, P. Tso, M. Liu, and W. S. Davidson Modulation of Apolipoprotein A-IV Lipid Binding by an Interaction between the N and C Termini J. Biol. Chem., September 28, 2007; 282(39): 28385 - 28394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Hanniman, G. Lambert, Y. Inoue, F. J. Gonzalez, and C. J. Sinal Apolipoprotein A-IV is regulated by nutritional and metabolic stress: involvement of glucocorticoids, HNF-4{alpha}, and PGC-1{alpha} J. Lipid Res., November 1, 2006; 47(11): 2503 - 2514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liang, X.-C. Jiang, R. Liu, G. Liang, T. P. Beyer, H. Gao, T. P. Ryan, S. Dan Li, P. I. Eacho, and G. Cao Liver X Receptors (LXRs) Regulate Apolipoprotein AIV-Implications of the Antiatherosclerotic Effect of LXR Agonists Mol. Endocrinol., August 1, 2004; 18(8): 2000 - 2010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ezeh, M. Haiman, H. F. Alber, B. Kunz, B. Paulweber, A. Lingenhel, H.-G. Kraft, F. Weidinger, O. Pachinger, H. Dieplinger, et al. Plasma distribution of apoA-IV in patients with coronary artery disease and healthy controls J. Lipid Res., August 1, 2003; 44(8): 1523 - 1529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ferrer, E. Bigot-Corbel, P. N'Guyen, M. Krempf, and J.-M. Bard Quantitative Measurement of Lipoprotein Particles Containing Both Apolipoprotein AIV and Apolipoprotein B in Human Plasma by a Noncompetitive ELISA Clin. Chem., June 1, 2002; 48(6): 884 - 890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Verges, B. Guerci, V. Durlach, C. Galland-Jos, J. L. Paul, L. Lagrost, and P. Gambert Increased plasma apoA-IV level is a marker of abnormal postprandial lipemia: a study in normoponderal and obese subjects J. Lipid Res., December 1, 2001; 42(12): 2021 - 2029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Sun, F. K Welty, G. G Dolnikowski, A. H Lichtenstein, and E. J Schaefer Effects of a National Cholesterol Education Program Step II Diet on apolipoprotein A-IV metabolism within triacylglycerol-rich lipoproteins and plasma Am. J. Clinical Nutrition, September 1, 2001; 74(3): 308 - 314. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Kronenberg, M. Stuhlinger, E. Trenkwalder, F. S. Geethanjali, O. Pachinger, A. von Eckardstein, and H. Dieplinger Low apolipoprotein A-IV plasma concentrations in men with coronary artery disease J. Am. Coll. Cardiol., September 1, 2000; 36(3): 751 - 757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Fournier, V. Atger, J.-L. Paul, M. Sturm, N. Duverger, G. H. Rothblat, and N. Moatti Human ApoA-IV Overexpression in Transgenic Mice Induces cAMP-Stimulated Cholesterol Efflux From J774 Macrophages to Whole Serum Arterioscler. Thromb. Vasc. Biol., May 1, 2000; 20(5): 1283 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jansen, J. Lopez-Miranda, J. Salas, J. M. Ordovas, P. Castro, C. Marin, M. A. Ostos, F. Lopez-Segura, J. A. Jimenez-Pereperez, A. Blanco, et al. Effect of 347-Serine Mutation in Apoprotein A-IV on Plasma LDL Cholesterol Response to Dietary Fat Arterioscler. Thromb. Vasc. Biol., August 1, 1997; 17(8): 1532 - 1538. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. J. Kalogeris, M.-D. Rodriguez, and P. Tso Control of Synthesis and Secretion of Intestinal Apolipoprotein A-IV by Lipid J. Nutr., March 1, 1997; 127(3): 537 - 537. [Abstract] [Full Text]
|
|
|
|
|
|
A. von Eckardstein, Y. Huang, S. Wu, A. S. Sarmadi, S. Schwarz, A. Steinmetz, and G. Assmann Lipoproteins Containing Apolipoprotein A-IV but Not Apolipoprotein A-I Take Up and Esterify Cell-Derived Cholesterol in Plasma Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1755 - 1763. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
