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Journal of Lipid Research, Vol 30, 1545-1550, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

Absorption, storage, and distribution of beta-carotene in normal and beta-carotene-fed rats: roles of parenchymal and stellate cells

MR Lakshman, KA Asher, MG Attlesey, S Satchithanandam, I Mychkovsky and PJ Coutlakis
Lipid Research Laboratory, Veterans Administration Medical Center, Washington, DC 20422.

Absorption and storage of [14C]beta-carotene in control and beta- carotene-fed (BC-fed) rats were determined. Pre-feeding with beta- carotene for 2 weeks caused a 1.9-fold stimulation of its own absorption as well as its conversion to retinyl esters, whereas the absorption of [3H]retinyl acetate was unaffected. The liver and the lungs accounted for 60% and 30%, respectively, of the total recovered 14C radioactivity in both control and BC-fed groups. Beta-carotene accounted for 80-87% of the recovered 14C radioactivity in both the liver and the lung. Subcellular distribution of [14C]beta-carotene in both control and BC-fed groups revealed that the cytosol was the major fraction accounting for 44.4% and 26.8% of the radioactivity in the liver and lungs, respectively. Distribution of beta-carotene among liver parenchymal (PC) and stellate cells (STC) was determined in the two groups. Based on radioactivity, the PC and STC contained 22% and 78% of the total, respectively, in the control group; the corresponding values for the PC and STC in the BC-fed group were 48% and 52% of the total radioactivity, respectively. Based on the beta-carotene concentration following chronic beta-carotene feeding, PC contained 75.5% while the STC had 24.5% of the total beta-carotene. Thus, parenchymal cells seem to be the major hepatic storage site for dietary beta-carotene after chronic feeding.
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