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Journal of Lipid Research, Vol 30, 1811-1817, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

Contribution of 3-hydroxyisobutyrate to the measurement of 3- hydroxybutyrate in human plasma: comparison of enzymatic and gas-liquid chromatography-mass spectrometry assays in normal and in diabetic subjects

A Avogaro and DM Bier
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.

In this study we employed a capillary gas-liquid chromatographic-mass spectrometric (GLC-MS) method to measure the plasma concentrations of 3- hydroxybutyrate (3-OHB) and 3-hydroxyisobutyrate (3-OHIB) in overnight fasted diabetic subjects and in normal subjects. Plasma contents of 3- hydroxybutyrate measured in this fashion were identical to those obtained by enzymatic assay using a commercial preparation of beta- hydroxybutyrate dehydrogenase, indicating no significant contamination of this enzyme preparation with 3-hydroxyisobutyrate dehydrogenase. In normal individuals, plasma 3-OHIB concentration was 21 +/- 2 microM in the overnight fasted state and was higher in diabetic subjects (38 +/- 5 microM) and in subjects fasted for 72 h (97 +/- 4 microM). In the postabsorptive state, 3-OHIB was 33% the concentration of 3-OHB in normals and 17% that of 3-OHB in the diabetics.
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