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Journal of Lipid Research, Vol 30, 1889-1893, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

Identification of bile alcohols in rat bile

T Hiraoka, T Kohda, D Kosaka, T Yamauchi, K Kihira, T Kuramoto, T Hoshita and G Kajiyama
First Department of Internal Medicine, Hiroshima University, School of Medicine, Japan.

Bile alcohols in rat bile were analyzed by gas-liquid chromatography- mass spectrometry. Six bile alcohols were newly identified as minor constituents in addition to 5 beta-cholestane-3 alpha,7 alpha,12 alpha,26-tetrol, major bile alcohol of rat bile. The bile alcohols newly identified were 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol, 5 alpha-cholestane-3 alpha,7 alpha,12 alpha,26- tetrol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,26-pentol, 5 alpha-cholestane-3 alpha,7 alpha,12 alpha,25,26-pentol, 5 beta- cholestane-3 alpha,7 alpha,12 alpha,25,26-pentol, and 5 beta-cholestane- 3 alpha,6 beta,7 beta,25,26-pentol. The biliary bile alcohols of the rat occurred mainly as the sulfuric acid esters and, in lesser amounts, as glucuronoconjugated and unconjugated forms. The amount of total bile alcohols was about 27.9 nmol in 1 ml of bile.
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