J. Lipid Res. Acyl Labeled PIP's available August 1, 2008
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Journal of Lipid Research, Vol 30, 1983-1986, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

A fast atom bombardment-mass spectrometric method to quantitate lysophosphatidylserine in rat brain

E Benfenati, G De Bellis, S Chen, L Bettazzoli, R Fanelli, MT Tacconi, G Kirschner and G Pege
Istituto di Ricerche Farmacologiche Mario Negri, Bergamo, Italy.

A method to quantitate lysophosphatidylserine by fast atom bombardment- mass spectrometry using 1-hexa-decanoyl-sn-glycero-3-phospho-L-serine as internal standard is described. The standard curve is linear with a correlation coefficient r2 = 0.999 from 10 to 1000 ng. This curve has been used to quantitate LPS in rat brain using phosphorus assay as a test control. We found 475 +/- 70 ng of LPS in 1 mg of tissue (n = 3). This method presents advantages due to its sensitivity and its capability to give molecular information of the unmodified compound.
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