Journal of Lipid Research, Vol 30, 1987-1995, Copyright © 1989 by Lipid Research, Inc.
A simple culture method of fat cells from mature fat tissue fragments
H Sugihara, S Funatsumaru, N Yonemitsu, S Miyabara, S Toda and Y Hikichi
Department of Pathology, Saga Medical School, Japan.
To obtain immature fat cells in vitro, we used a primary culture of
undigested mature fat tissue fragments. The immature fat cells, i.e.,
fibroblast-like fat cells, proliferated extensively from the fat tissue and
differentiated after reaching confluence. The process of differentiation
was assumed by the development of intracytoplasmic lipid droplets and by
the triglyceride content in the cells. Cellular differentiation was induced
in high percentages (over 70-80%) of the cells in the medium containing
high glucose concentrations (200 mg/dl) supplemented with 10-20% newborn
calf serum. The intracellular accumulation of triglyceride was also
enhanced by insulin administration. In these cells, a reciprocal
relationship was observed between proliferation and differentiation.
Fibroblast-like fat cells derived from mature fat tissue in this simple
culture system are suitable for the study of the proliferation and
differentiation of immature fat cells.
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