Journal of Lipid Research, Vol 30, 141-148, Copyright © 1989 by Lipid Research, Inc.
Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact
HF Zhang, WB Davis, XS Chen, RL Whisler and DG Cornwell
Department of Physiological Chemistry, Ohio State University, Columbus, 43210.
Low density lipoproteins (LDL), isolated by ultracentrifugal flotation,
were oxidized (LDLOXID) slowly during dialysis against 0.15 M NaCl and
subsequent incubation in 96% air-4% CO2 at 37 degrees C. Butylated
hydroxytoluene prevented LDL oxidation. LDL preparations from different
sera were oxidized at different rates and the degree of lipid peroxidation
was controlled by varying the incubation time. Mild oxidation did not alter
the electrophoretic mobility of the LDLOXID preparations. LDLOXID contained
lipid peroxides in neutral lipids, had increased amounts of
lysophosphatidylcholine, and contained a number of complex oxidation
products that were generated from the oxidation of free fatty acids. These
oxidation products included large amounts of soluble material that
cross-reacted with antibodies to PGE2 but not 6- keto-PGF1 alpha. The
amount of cross-reacting material was proportional to the degree of lipid
peroxidation. Cross-reacting material in LDLOXID preparations was evidently
formed from the oxidation of free fatty acids released from LDL, since
cross-reacting material was also formed when a synthetic fat emulsion was
oxidized in the presence of free arachidonic acid.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
