HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Makover, A. Articles by Goodman, D. S. Search for Related Content PubMed PubMed Citation Articles by Makover, A. Articles by Goodman, D. S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 30, 171-180, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

Localization of retinol-binding protein messenger RNA in the rat kidney and in perinephric fat tissue

A Makover, DR Soprano, ML Wyatt and DS Goodman
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, NY 10032.

The cellular localization of retinol-binding protein (RBP) messenger RNA (mRNA) in the kidney, and the developmental pattern of the renal expression of the RBP gene, were studied in the Sprague-Dawley rat. In situ hybridization studies were conducted with single-stranded cRNA probes, using sections of adult and young rat kidneys. These studies revealed specific localization of RBP mRNA in the outer stripe of the medulla, specifically localized in the S3 segment of the proximal tubules. Northern blot analysis demonstrated that RBP mRNA was not detectable in the kidney before birth or during the first week postpartum, but was clearly detected by the end of the second week of age. No RBP mRNA was observed in the kidney by in situ hybridization at 12 days of age. At 26 days of age, however, RBP mRNA was clearly detected by the in situ hybridization technique, localized in the same anatomic region as that observed in the adult kidney. Transthyretin mRNA was not detected in the adult kidney. Previous studies have shown that immunoreactive RBP is localized in the convoluted segment of the proximal tubules of the rat kidney. The present results demonstrate that RBP mRNA in the kidney is localized in an anatomic region (the S3 segment of the proximal tubules) different from that of immunoreactive RBP. In addition, an intense RBP mRNA hybridization signal was detected in the perinephric fat tissue of 26- and 40-day-old and adult rats. Further analysis of RNA from epididymal fat showed a level of RBP mRNA approximately 20% of that of liver. The function of RBP synthesized in the kidney and adipose tissue remains to be determined. We have previously hypothesized that RBP synthesized in extrahepatic tissue may function in the recycling of retinol back to the liver or to other target tissues.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. MARINO, D. ANDREWS, D. BROWN, and R. T. McCLUSKEY Transcytosis of Retinol-Binding Protein across Renal Proximal Tubule Cells after Megalin (gp 330)-Mediated Endocytosis J. Am. Soc. Nephrol., April 1, 2001; 12(4): 637 - 648. [Abstract] [Full Text] [PDF]

				E. I. CHRISTENSEN and T. E. WILLNOW Essential Role of Megalin in Renal Proximal Tubule for Vitamin Homeostasis J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2224 - 2236. [Full Text]

				E. I. CHRISTENSEN, J. O. MOSKAUG, H. VORUM, C. JACOBSEN, T. E. GUNDERSEN, A. NYKJ&Aelig;R, R. BLOMHOFF, T. E. WILLNOW, and S. K. MOESTRUP Evidence for an Essential Role of Megalin in Transepithelial Transport of Retinol J. Am. Soc. Nephrol., April 1, 1999; 10(4): 685 - 695. [Abstract] [Full Text]

				Y. Kawashima-Ohya, Y. Kuruta, W. Yan, T. Kawamoto, M. Noshiro, and Y. Kato Retinol-Binding Protein Is Produced by Rabbit Chondrocytes and Responds to Parathyroid Hormone (PTH)/PTH-Related Peptide-Cyclic Adenosine Monophosphate Pathway Endocrinology, March 1, 1999; 140(3): 1075 - 1081. [Abstract] [Full Text]

				S. Wei, K. Lai, S. Patel, R. Piantedosi, H. Shen, V. Colantuoni, F. B. Kraemer, and W. S. Blaner Retinyl Ester Hydrolysis and Retinol Efflux from BFC-1beta Adipocytes J. Biol. Chem., May 30, 1997; 272(22): 14159 - 14165. [Abstract] [Full Text] [PDF]

				R Blomhoff, M. Green, T Berg, and K. Norum Transport and storage of vitamin A Science, October 19, 1990; 250(4979): 399 - 404. [Abstract] [PDF]

				M. M. Sousa, A. G. W. Norden, C. Jacobsen, T. E. Willnow, E. I. Christensen, R. V. Thakker, P. J. Verroust, S. K. Moestrup, and M. J. Saraiva Evidence for the Role of Megalin in Renal Uptake of Transthyretin J. Biol. Chem., December 1, 2000; 275(49): 38176 - 38181. [Abstract] [Full Text] [PDF]