Journal of Lipid Research, Vol 30, 521-528, Copyright © 1989 by Lipid Research, Inc.
Proton magnetic resonance spectroscopy of plasma from patients with dyslipoproteinemia: identification of factors governing methyl and methylene proton line widths
FG Herring, PS Phillips and PH Pritchard
Department of Chemistry, University of British Columbia, Vancouver, Canada.
The line width of the proton magnetic resonance spectrum (MRS) of the
composite methylene and methyl resonances of plasma has been reported as a
marker for the presence of malignancy. In this study, the contribution of
very low density (VLDL), low density (LDL), and high density lipoproteins
(HDL) to the MRS line width was determined. This was achieved by measuring
the MRS line widths for the plasma from patients with primary disorders of
lipoprotein metabolism and from normal individuals. A negative correlation
between plasma trigylceride levels and the average line width was observed
and this was confirmed in normal plasma to which pure VLDL was added. Also,
computer simulations were employed to demonstrate how the line width varies
in such complex mixtures of lipoproteins. We demonstrate that the line
width is governed by the relative contribution of VLDL and HDL to the
composite line shape. This is particularly important when the shoulder from
the HDL line lies near the half-height of the VLDL line. As changes in
VLDL/HDL ratio occur in patients with malignancy, we propose that this is
the basis of the narrowed MRS lines observed in the proposed test for
malignancy. However, any individual with elevated VLDL will be false
positive in this test.
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