Journal of Lipid Research, Vol 30, 616-627, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

Separation of derivatized glycosphingolipids into individual molecular species by high performance liquid chromatography

H Kadowaki, KE Rys-Sikora and RS Koff
Department of Medicine, Framingham Union Hospital, MA 01701.

The high performance liquid chromatography separation of the perbenzoyl derivatives of the neutral glycosphingolipids (GlcCer, LacCer, GbOse3Cer, GbOse4Cer, and GgOse3Cer) and the p-bromophenacyl and 2,4- dinitrophenyl hydrazide derivatives of the gangliosides (GM4, GM3, GM2, GM1, GD1a) into individual molecular species on a C18 reversed-phase column is described. Peaks were identified by comparing their relative retention times to the relative retention time of the corresponding glycosphingolipid of known molecular species composition. As little as 5 to 10 pmol of each molecular species of neutral glycosphingolipids and 3 to 5 pmol of the gangliosides can be detected. The effects of changes in the proportion of acetonitrile, methanol, and water in the mobile phase and of column temperature on the molecular species separation are described. A procedure for the tentative identification of glycosphingolipid molecular species based on their relative retention times is presented.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				X. Han and H. Cheng Characterization and direct quantitation of cerebroside molecular species from lipid extracts by shotgun lipidomics J. Lipid Res., January 1, 2005; 46(1): 163 - 175. [Abstract] [Full Text] [PDF]