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Journal of Lipid Research, Vol 30, 627-630, Copyright © 1989 by Lipid Research, Inc.


ARTICLES

A spectrophotometric assay for lipid peroxides in serum lipoproteins using a commercially available reagent

M el-Saadani, H Esterbauer, M el-Sayed, M Goher, AY Nassar and G Jurgens
Institute of Medical Biochemistry, Graz, Austria.

A method is described for measuring lipid peroxides by means of the color reagent of a commercially available test kit for cholesterol estimation. In principle, this assay makes use of the oxidative capacity of lipid peroxides to convert iodide to iodine, which can be measured photometrically at 365 nm. Calibration curves were obtained using peroxides such as H2O2, t-butyl hydroperoxide, and cumene hydroperoxide. A stoichiometric relationship was observed between the amount of organic peroxides assayed and the concentration of iodine produced. Concentrations of lipid peroxides as small as 1 nmol/ml could be measured. The ability to estimate lipid peroxides of isolated low density lipoprotein was demonstrated.
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Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 1939 - 1946.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
V. A. Folcik, R. Aamir, and M. K. Cathcart
Cytokine Modulation of LDL Oxidation by Activated Human Monocytes
Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 1954 - 1961.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
S.R. Li, Q. Yang, E. Koller, A. Kurtaran, C. Bischof, M. Leimer, F. Rauscha, J. Pidlich, and I. Virgolini
Modified LDL Decreases the Binding of Prostaglandin E2, I2, and E1 Onto Monocytes in Patients With Peripheral Vascular Disease
Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 2066 - 2073.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
S. Devaraj, B. Adams-Huet, C.J. Fuller, and I. Jialal
Dose-Response Comparison of RRR-{alpha}-Tocopherol and All-Racemic {alpha}-Tocopherol on LDL Oxidation
Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 2273 - 2279.
[Abstract] [Full Text]


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J. Biol. Chem.Home page
K. Oorni, M. O. Pentikainen, A. Annila, and P. T. Kovanen
Oxidation of Low Density Lipoprotein Particles Decreases Their Ability to Bind to Human Aortic Proteoglycans. DEPENDENCE ON OXIDATIVE MODIFICATION OF THE LYSINE RESIDUES
J. Biol. Chem., August 22, 1997; 272(34): 21303 - 21311.
[Abstract] [Full Text] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
D. Stengel, M. Antonucci, M. Arborati, D. Hourton, S. Griglio, M. J. Chapman, and E. Ninio
Expression of the PAF Receptor in Human Monocyte–Derived Macrophages Is Downregulated by Oxidized LDL: Relevance to the Inflammatory Phase of Atherogenesis
Arterioscler. Thromb. Vasc. Biol., May 1, 1997; 17(5): 954 - 962.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
P. Lesnik, C. Dachet, L. Petit, M. Moreau, S. Griglio, P. Brudi, and M. J. Chapman
Impact of a Combination of a Calcium Antagonist and a ß-Blocker on Cell- and Copper-Mediated Oxidation of LDL and on the Accumulation and Efflux of Cholesterol in Human Macrophages and Murine J774 Cells
Arterioscler. Thromb. Vasc. Biol., May 1, 1997; 17(5): 979 - 988.
[Abstract] [Full Text]


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CirculationHome page
V. Deckert, L. Persegol, L. Viens, G. Lizard, A. Athias, C. Lallemant, P. Gambert, and L. Lagrost
Inhibitors of Arterial Relaxation Among Components of Human Oxidized Low-Density Lipoproteins: Cholesterol Derivatives Oxidized in Position 7 Are Potent Inhibitors of Endothelium-Dependent Relaxation
Circulation, February 4, 1997; 95(3): 723 - 731.
[Abstract] [Full Text]


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J. Biol. Chem.Home page
Q. Li and M. K. Cathcart
Selective Inhibition of Cytosolic Phospholipase A2 in Activated Human Monocytes. REGULATION OF SUPEROXIDE ANION PRODUCTION AND LOW DENSITY LIPOPROTEIN OXIDATION
J. Biol. Chem., January 24, 1997; 272(4): 2404 - 2411.
[Abstract] [Full Text] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
M. Kusuhara, A. Chait, A. Cader, and B. C. Berk
Oxidized LDL Stimulates Mitogen-Activated Protein Kinases in Smooth Muscle Cells and Macrophages
Arterioscler. Thromb. Vasc. Biol., January 1, 1997; 17(1): 141 - 148.
[Abstract] [Full Text]


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Circ. Res.Home page
S. Rangaswamy, M. S. Penn, G. M. Saidel, and G. M. Chisolm
Exogenous Oxidized Low-Density Lipoprotein Injures and Alters the Barrier Function of Endothelium in Rats In Vivo
Circ. Res., January 1, 1997; 80(1): 37 - 44.
[Abstract] [Full Text]


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J. Biol. Chem.Home page
S. L. Hazen, F. F. Hsu, K. Duffin, and J. W. Heinecke
Molecular Chlorine Generated by the Myeloperoxidase-Hydrogen Peroxide-Chloride System of Phagocytes Converts Low Density Lipoprotein Cholesterol into a Family of Chlorinated Sterols
J. Biol. Chem., September 20, 1996; 271(38): 23080 - 23088.
[Abstract] [Full Text] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
M. D. P. Navazo, L. Daviet, E. Ninio, and J. L. McGregor
Identification on Human CD36 of a Domain (155-183) Implicated in Binding Oxidized Low-Density Lipoproteins (Ox-LDL)
Arterioscler. Thromb. Vasc. Biol., August 1, 1996; 16(8): 1033 - 1039.
[Abstract] [Full Text]


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J. Biol. Chem.Home page
C. K. Mukhopadhyay, E. Ehrenwald, and P. L. Fox
Ceruloplasmin Enhances Smooth Muscle Cell- and Endothelial Cell-mediated Low Density Lipoprotein Oxidation by a Superoxide-dependent Mechanism
J. Biol. Chem., June 21, 1996; 271(25): 14773 - 14778.
[Abstract] [Full Text] [PDF]


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Arterioscler. Thromb. Vasc. Bio.Home page
A. Stiko, J. Regnstrom, P. K. Shah, B. Cercek, and J. Nilsson
Active Oxygen Species and Lysophosphatidylcholine Are Involved in Oxidized Low Density Lipoprotein Activation of Smooth Muscle Cell DNA Synthesis
Arterioscler. Thromb. Vasc. Biol., February 1, 1996; 16(2): 194 - 200.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
S. Keidar, M. Kaplan, and M. Aviram
Angiotensin II–Modified LDL Is Taken Up by Macrophages Via the Scavenger Receptor, Leading to Cellular Cholesterol Accumulation
Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 97 - 105.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
M. P.S. Ares, B. Kallin, P. Eriksson, and J. Nilsson
Oxidized LDL Induces Transcription Factor Activator Protein–1 but Inhibits Activation of Nuclear Factor–{kappa}B in Human Vascular Smooth Muscle Cells
Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1584 - 1590.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
A. D. Tselepis, C. Dentan, S.-A. P. Karabina, M. J. Chapman, and E. Ninio
PAF-Degrading Acetylhydrolase Is Preferentially Associated With Dense LDL and VHDL-1 in Human Plasma : Catalytic Characteristics and Relation to the Monocyte-Derived Enzyme
Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1764 - 1773.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
I. Maor, H. Mandel, and M. Aviram
Macrophage Uptake of Oxidized LDL Inhibits Lysosomal Sphingomyelinase, Thus Causing the Accumulation of Unesterified Cholesterol–Sphingomyelin–Rich Particles in the Lysosomes : A Possible Role for 7-Ketocholesterol
Arterioscler. Thromb. Vasc. Biol., September 1, 1995; 15(9): 1378 - 1387.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
P. Lesnik, C. Dentan, A. Vonica, M. Moreau, and M. J. Chapman
Tissue Factor Pathway Inhibitor Activity Associated With LDL Is Inactivated by Cell- and Copper-Mediated Oxidation
Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1121 - 1130.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
T. M. Stulnig, H. Klocker, H. J. Harwood Jr, G. Jurgens, D. Schonitzer, E. Jarosch, L. A. Huber, A. Amberger, and G. Wick
In Vivo LDL Receptor and HMG-CoA Reductase Regulation in Human Lymphocytes and Its Alterations During Aging
Arterioscler. Thromb. Vasc. Biol., July 1, 1995; 15(7): 872 - 878.
[Abstract] [Full Text]


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Arterioscler. Thromb. Vasc. Bio.Home page
A. Hammer, G. Kager, G. Dohr, H. Rabl, I. Ghassempur, and G. Jurgens
Generation, Characterization, and Histochemical Application of Monoclonal Antibodies Selectively Recognizing Oxidatively Modified ApoB-Containing Serum Lipoproteins
Arterioscler. Thromb. Vasc. Biol., May 1, 1995; 15(5): 704 - 713.
[Abstract] [Full Text]


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J. Biol. Chem.Home page
D. I. Draganov, P. L. Stetson, C. E. Watson, S. S. Billecke, and B. N. La Du
Rabbit Serum Paraoxonase 3 (PON3) Is a High Density Lipoprotein-associated Lactonase and Protects Low Density Lipoprotein against Oxidation
J. Biol. Chem., October 20, 2000; 275(43): 33435 - 33442.
[Abstract] [Full Text] [PDF]




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