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Journal of Lipid Research, Vol 30, 627-630, Copyright © 1989 by Lipid Research, Inc.
M el-Saadani, H Esterbauer, M el-Sayed, M Goher, AY Nassar and G Jurgens
A method is described for measuring lipid peroxides by means of the color
reagent of a commercially available test kit for cholesterol estimation. In
principle, this assay makes use of the oxidative capacity of lipid
peroxides to convert iodide to iodine, which can be measured
photometrically at 365 nm. Calibration curves were obtained using peroxides
such as H2O2, t-butyl hydroperoxide, and cumene hydroperoxide. A
stoichiometric relationship was observed between the amount of organic
peroxides assayed and the concentration of iodine produced. Concentrations
of lipid peroxides as small as 1 nmol/ml could be measured. The ability to
estimate lipid peroxides of isolated low density lipoprotein was
demonstrated.
ARTICLES
A spectrophotometric assay for lipid peroxides in serum lipoproteins using a commercially available reagent
Institute of Medical Biochemistry, Graz, Austria.
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P. Lesnik, C. Dachet, L. Petit, M. Moreau, S. Griglio, P. Brudi, and M. J. Chapman Impact of a Combination of a Calcium Antagonist and a ß-Blocker on Cell- and Copper-Mediated Oxidation of LDL and on the Accumulation and Efflux of Cholesterol in Human Macrophages and Murine J774 Cells Arterioscler. Thromb. Vasc. Biol., May 1, 1997; 17(5): 979 - 988. [Abstract] [Full Text]
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V. Deckert, L. Persegol, L. Viens, G. Lizard, A. Athias, C. Lallemant, P. Gambert, and L. Lagrost Inhibitors of Arterial Relaxation Among Components of Human Oxidized Low-Density Lipoproteins: Cholesterol Derivatives Oxidized in Position 7 Are Potent Inhibitors of Endothelium-Dependent Relaxation Circulation, February 4, 1997; 95(3): 723 - 731. [Abstract] [Full Text]
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Q. Li and M. K. Cathcart Selective Inhibition of Cytosolic Phospholipase A2 in Activated Human Monocytes. REGULATION OF SUPEROXIDE ANION PRODUCTION AND LOW DENSITY LIPOPROTEIN OXIDATION J. Biol. Chem., January 24, 1997; 272(4): 2404 - 2411. [Abstract] [Full Text] [PDF]
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M. Kusuhara, A. Chait, A. Cader, and B. C. Berk Oxidized LDL Stimulates Mitogen-Activated Protein Kinases in Smooth Muscle Cells and Macrophages Arterioscler. Thromb. Vasc. Biol., January 1, 1997; 17(1): 141 - 148. [Abstract] [Full Text]
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S. Rangaswamy, M. S. Penn, G. M. Saidel, and G. M. Chisolm Exogenous Oxidized Low-Density Lipoprotein Injures and Alters the Barrier Function of Endothelium in Rats In Vivo Circ. Res., January 1, 1997; 80(1): 37 - 44. [Abstract] [Full Text]
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S. L. Hazen, F. F. Hsu, K. Duffin, and J. W. Heinecke Molecular Chlorine Generated by the Myeloperoxidase-Hydrogen Peroxide-Chloride System of Phagocytes Converts Low Density Lipoprotein Cholesterol into a Family of Chlorinated Sterols J. Biol. Chem., September 20, 1996; 271(38): 23080 - 23088. [Abstract] [Full Text] [PDF]
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M. D. P. Navazo, L. Daviet, E. Ninio, and J. L. McGregor Identification on Human CD36 of a Domain (155-183) Implicated in Binding Oxidized Low-Density Lipoproteins (Ox-LDL) Arterioscler. Thromb. Vasc. Biol., August 1, 1996; 16(8): 1033 - 1039. [Abstract] [Full Text]
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C. K. Mukhopadhyay, E. Ehrenwald, and P. L. Fox Ceruloplasmin Enhances Smooth Muscle Cell- and Endothelial Cell-mediated Low Density Lipoprotein Oxidation by a Superoxide-dependent Mechanism J. Biol. Chem., June 21, 1996; 271(25): 14773 - 14778. [Abstract] [Full Text] [PDF]
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A. Stiko, J. Regnstrom, P. K. Shah, B. Cercek, and J. Nilsson Active Oxygen Species and Lysophosphatidylcholine Are Involved in Oxidized Low Density Lipoprotein Activation of Smooth Muscle Cell DNA Synthesis Arterioscler. Thromb. Vasc. Biol., February 1, 1996; 16(2): 194 - 200. [Abstract] [Full Text]
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S. Keidar, M. Kaplan, and M. Aviram Angiotensin II–Modified LDL Is Taken Up by Macrophages Via the Scavenger Receptor, Leading to Cellular Cholesterol Accumulation Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 97 - 105. [Abstract] [Full Text]
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M. P.S. Ares, B. Kallin, P. Eriksson, and J. Nilsson Oxidized LDL Induces Transcription Factor Activator Protein–1 but Inhibits Activation of Nuclear Factor–{kappa}B in Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1584 - 1590. [Abstract] [Full Text]
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A. D. Tselepis, C. Dentan, S.-A. P. Karabina, M. J. Chapman, and E. Ninio PAF-Degrading Acetylhydrolase Is Preferentially Associated With Dense LDL and VHDL-1 in Human Plasma : Catalytic Characteristics and Relation to the Monocyte-Derived Enzyme Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1764 - 1773. [Abstract] [Full Text]
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I. Maor, H. Mandel, and M. Aviram Macrophage Uptake of Oxidized LDL Inhibits Lysosomal Sphingomyelinase, Thus Causing the Accumulation of Unesterified Cholesterol–Sphingomyelin–Rich Particles in the Lysosomes : A Possible Role for 7-Ketocholesterol Arterioscler. Thromb. Vasc. Biol., September 1, 1995; 15(9): 1378 - 1387. [Abstract] [Full Text]
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P. Lesnik, C. Dentan, A. Vonica, M. Moreau, and M. J. Chapman Tissue Factor Pathway Inhibitor Activity Associated With LDL Is Inactivated by Cell- and Copper-Mediated Oxidation Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1121 - 1130. [Abstract] [Full Text]
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T. M. Stulnig, H. Klocker, H. J. Harwood Jr, G. Jurgens, D. Schonitzer, E. Jarosch, L. A. Huber, A. Amberger, and G. Wick In Vivo LDL Receptor and HMG-CoA Reductase Regulation in Human Lymphocytes and Its Alterations During Aging Arterioscler. Thromb. Vasc. Biol., July 1, 1995; 15(7): 872 - 878. [Abstract] [Full Text]
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A. Hammer, G. Kager, G. Dohr, H. Rabl, I. Ghassempur, and G. Jurgens Generation, Characterization, and Histochemical Application of Monoclonal Antibodies Selectively Recognizing Oxidatively Modified ApoB-Containing Serum Lipoproteins Arterioscler. Thromb. Vasc. Biol., May 1, 1995; 15(5): 704 - 713. [Abstract] [Full Text]
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D. I. Draganov, P. L. Stetson, C. E. Watson, S. S. Billecke, and B. N. La Du Rabbit Serum Paraoxonase 3 (PON3) Is a High Density Lipoprotein-associated Lactonase and Protects Low Density Lipoprotein against Oxidation J. Biol. Chem., October 20, 2000; 275(43): 33435 - 33442. [Abstract] [Full Text] [PDF]
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