Journal of Lipid Research, Vol 30, 761-764, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

Separation of phosphatidylinositols and other phospholipids by two-step one-dimensional thin-layer chromatography

JD Medh and PH Weigel
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

A quick and efficient thin-layer chromatographic procedure is described for the separation of phosphatidylinositol-4,5-bisphosphate, phosphatidylinositol-4-monophosphate, phosphatidylinositol, phosphatidylcholine, phosphatidic acid, and phosphatidylethanolamine. The method involves development of the phospholipids successively in two different solvent systems but in the same direction. The method is simple, reproducible, and gives good resolution of the six different phospholipids tested. About 8-10 32P-labeled phospholipids isolated from rat hepatocytes were separated by this method; the six mentioned above were identified. Thus, the technique has potential application for both qualitative and quantitative analysis of phospholipid mixtures, such as the phosphoinositides, in cell or tissue extracts.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Liu, P. Oh, T. Horner, R. A. Rogers, and J. E. Schnitzer Organized Endothelial Cell Surface Signal Transduction in Caveolae Distinct from Glycosylphosphatidylinositol-anchored Protein Microdomains J. Biol. Chem., March 14, 1997; 272(11): 7211 - 7222. [Abstract] [Full Text] [PDF]