HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kondo, K. Articles by Fidge, N. Search for Related Content PubMed PubMed Citation Articles by Kondo, K. Articles by Fidge, N. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 30, 939-944, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

Quantitation of apolipoprotein A-IV in human plasma using a competitive enzyme-linked immunosorbent assay

K Kondo, C Allan and N Fidge
Baker Medical Research Institute, Melbourne, Victoria, Australia.

A method for measuring human apolipoprotein A-IV has been developed using the competitive enzyme-linked immunosorbent assay (ELISA) system. The assay described is relatively easy, rapid, and inexpensive to perform, uses convenient dilutions of plasma (1/8-1/32) but is sensitive enough to quantitate the apoA-IV content of lipoproteins following gel filtration of small (0.3-0.5 ml) volumes of plasma. The working range is 100-600 ng of apoA-IV per 50-microliters sample and the intra- and interassay coefficients of variations are 7.5 and 10.2% (means), respectively. The mean apoA-IV concentration of 100 subjects was found to be 16.4 +/- 5.4 mg/dl. The assay can be performed on untreated plasma samples which may be stored frozen (-20 degrees C) for up to 2 months.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Anderson Candidate-based proteomics in the search for biomarkers of cardiovascular disease J. Physiol., February 15, 2005; 563(1): 23 - 60. [Abstract] [Full Text] [PDF]

				F. Ferrer, E. Bigot-Corbel, P. N'Guyen, M. Krempf, and J.-M. Bard Quantitative Measurement of Lipoprotein Particles Containing Both Apolipoprotein AIV and Apolipoprotein B in Human Plasma by a Noncompetitive ELISA Clin. Chem., June 1, 2002; 48(6): 884 - 890. [Abstract] [Full Text] [PDF]

				L. Vergnes, N. Baroukh, M. A. Ostos, G. Castro, N. Duverger, M. N. Nanjee, J. Najib, J.-C. Fruchart, N. E. Miller, M. M. Zakin, et al. Expression of Human Apolipoprotein A-I/C-III/A-IV Gene Cluster in Mice Induces Hyperlipidemia but Reduces Atherogenesis Arterioscler. Thromb. Vasc. Biol., October 1, 2000; 20(10): 2267 - 2274. [Abstract] [Full Text] [PDF]

				T. J. Kalogeris, M.-D. Rodriguez, and P. Tso Control of Synthesis and Secretion of Intestinal Apolipoprotein A-IV by Lipid J. Nutr., March 1, 1997; 127(3): 537 - 537. [Abstract] [Full Text]