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Journal of Lipid Research, Vol 30, 944-949, Copyright © 1989 by Lipid Research, Inc.

ARTICLES

Purification of glycerol dialkyl nonitol tetraether from Sulfolobus acidocaldarius

SL Lo, CE Montague and EL Chang
Geo-Centers, Newton Upper Falls, MA.

A modified procedure for extraction and purification of hydrolyzed archaebacterial lipids is described. Lipids were extracted from Sulfolobus acidocaldarius using a Soxhlet extraction procedure followed by trichloroacetic acid solvent-extraction of the residue. The yield of total extractable material by this protocol was 14% which, after a two- phase wash, yielded 10% lipid. Modifications to the published steps for purifying the subsequently hydrolyzed lipids were developed to purify glycerol dialkyl nonitol tetraether (GDNT). The nearly colorless final macrocyclic product was characterized by TLC, IR, NMR, and mass spectrometry.
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