HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baer, A. N. Articles by Green, F. A. Search for Related Content PubMed PubMed Citation Articles by Baer, A. N. Articles by Green, F. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 31, 125-130, Copyright © 1990 by Lipid Research, Inc.

ARTICLES

Free and esterified 13(R,S)-hydroxyoctadecadienoic acids: principal oxygenase products in psoriatic skin scales

AN Baer, PB Costello and FA Green
Department of Medicine, State University of New York, Buffalo.

Characterization of the chemical form and stereo-specificity of the fatty acid derivatives of arachidonic and linoleic acid in psoriatic epidermis is needed to define the enzymatic origin of these compounds and their possible role in pathogenesis. In an analysis of psoriatic skin scales, both free and esterified 13-hydroxyoctadecadienoic acids were the principal fatty acid derivatives, present in mean concentrations of 115 and 17 ng/mg scales, respectively. The analysis included reversed-phase high performance liquid chromatography of the free acid and of its methyl ester, gas chromatography, gas-liquid chromatography-mass spectrometry of the methyl ester derivatives, and chiral separation. The free and esterified 13-hydroxyoctadecadienoic acids isolated from the psoriatic scales contained a mixture of the S/R stereoisomers, averaging 1.9:1 for free 13- hydroxyoctadecadienoic acid. These findings are not compatible with the strict S- stereospecificity for oxygen insertion exhibited by mammalian lipoxygenase but rather could point to the action of a cyclooxygenase. The demonstration that a hydroxylated fatty acid derivative is esterified in vivo in psoriatic keratinocytes suggests that the physiology of these cells may be altered early in the process of keratinization.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. Chiba, H. Michibata, K. Wakimoto, M. Seishima, S. Kawasaki, K. Okubo, H. Mitsui, H. Torii, and Y. Imai Cloning of a Gene for a Novel Epithelium-specific Cytosolic Phospholipase A2, cPLA2{delta}, Induced in Psoriatic Skin J. Biol. Chem., March 26, 2004; 279(13): 12890 - 12897. [Abstract] [Full Text] [PDF]

				M. G. Malkowski, E. D. Thuresson, K. M. Lakkides, C. J. Rieke, R. Micielli, W. L. Smith, and R. M. Garavito Structure of Eicosapentaenoic and Linoleic Acids in the Cyclooxygenase Site of Prostaglandin Endoperoxide H Synthase-1 J. Biol. Chem., September 28, 2001; 276(40): 37547 - 37555. [Abstract] [Full Text] [PDF]