Journal of Lipid Research, Vol 31, 1965-1972, Copyright © 1990 by Lipid Research, Inc.
Antioxidants and resistance against oxidation of porcine LDL subfractions
G Knipping, M Rotheneder, G Striegl and H Esterbauer
Institute of Medical Biochemistry, University of Graz, Austria.
The objective of this study was to determine the level of antioxidants, the
content of fatty acids and peroxidation products, and the resistance
against oxidation of native porcine LDL1 and LDL2. There were no
significant differences in the fatty acid distribution of both native low
density lipoprotein (LDL) subfractions, which was similar to that of human
LDL. The total amount of alpha- and gamma-tocopherol of pig LDL was
significantly lower than in human LDL, and beta-carotene, lycopene, and
retinyl esters were totally absent. Levels of thiobarbituric acid-reacting
substances (TBARS) and lipid peroxides in freshly isolated pig LDL
subfractions were below or only slightly above the detection limit. The
susceptibility to oxidation of both LDL subfractions was investigated by
addition of Cu2+ as prooxidant. The results show that pig LDL subfractions
are much more susceptible to oxidation as measured by the duration of the
lag phase preceding the onset of rapid lipid peroxidation. From the low
content of vitamin E one would expect even much shorter lag phases. The
possibility therefore exists that pig LDL contains additional, and as yet
unidentified, antioxidants.
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