J. Lipid Res.
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Journal of Lipid Research, Vol 31, 2291-2293, Copyright © 1990 by Lipid Research, Inc.

ARTICLES

Preparative scale isolation of sphingosine

NS Radin
Mental Health Research Institute, University of Michigan, Ann Arbor 48109.

A high-yield method is described for the preparation of sphingenine from galactosylceramide. Two successive cleavage reactions are used, alkaline butanol to form galactosylsphingosine, then acidic acetonitrile to form the sphingenine. A column purification procedure utilizing silica gel is described. The method is superior to previously described methods in its rapidity and avoidance of isomerization and contamination by racemization and rearrangement products.
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Copyright © 1990 by the American Society for Biochemistry and Molecular Biology.