HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosenwald, A. G. Articles by Krag, S. S. Search for Related Content PubMed PubMed Citation Articles by Rosenwald, A. G. Articles by Krag, S. S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 31, 523-533, Copyright © 1990 by Lipid Research, Inc.

ARTICLES

Lec9 CHO glycosylation mutants are defective in the synthesis of dolichol

AG Rosenwald and SS Krag
Department of Biochemistry, Johns Hopkins School of Hygiene and Public Health, Baltimore, MD 21205.

Lec9 Chinese hamster ovary cells were found previously to be defective in the synthesis of N-linked glycans. This appeared to be the result of a defect in the synthesis of oligosaccharide lipid and lipid phosphate (Rosenwald, Stanley, and Krag. 1989. Mol. Cell. Biol. 9: 914-924). In this study we analyzed the steady state levels of long-chain polyisoprenyl lipids in Lec9 cells. We found that Lec9 cells are defective in the synthesis of dolichol. They accumulated a presumed precursor to dolichol, cis-a-unsaturated polyprenol and used this lipid in the synthesis of oligosaccharide lipid. Chain lengths of the activated polyprenols in Lec9 were the same lengths as dolichols in parental cells. Lec9 cells had increased levels of monosaccharylphosphoryl lipid and decreased levels of oligosaccharylpyrophosphoryl lipid compared to parental cells. The defect in Lec9 cells was specific for dolichol synthesis, since other aspects of [3H]mevalonate metabolism in Lec9 cells were the same as in parental cells. We hypothesize that Lec9 cells are defective in polyprenol reductase activity.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. A Lehrman Teaching dolichol-linked oligosaccharides more tricks with alternatives to metabolic radiolabeling Glycobiology, August 1, 2007; 17(8): 75R - 85R. [Abstract] [Full Text] [PDF]

				M Ermonval, R Cacan, K Gorgas, I. Haas, A Verbert, and G Buttin Differential fate of glycoproteins carrying a monoglucosylated form of truncated N-glycan in a new CHO line, MadIA214214, selected for a thermosensitive secretory defect J. Cell Sci., January 2, 1997; 110(3): 323 - 336. [Abstract] [PDF]