HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, H. F. Articles by Cornwell, D. G. Search for Related Content PubMed PubMed Citation Articles by Zhang, H. F. Articles by Cornwell, D. G. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 31, 551-565, Copyright © 1990 by Lipid Research, Inc.

ARTICLES

Effects of oxidized low density lipoproteins on arachidonic acid metabolism in smooth muscle cells

HF Zhang, WB Davis, XS Chen, KH Jones, RL Whisler and DG Cornwell
Department of Physiological Chemistry, Ohio State University, Columbus 43210.

The role of oxidized plasma lipoproteins in modifying arachidonic acid (AA) metabolism was studied in smooth muscle cells (SMC). Very low density lipoproteins (VLDL), unoxidized low density lipoproteins (LDLBHT) isolated with butylated hydroxytoluene (BHT), and oxidized LDL (LDLOXID) were separated from human serum. Thiobarbituric acid reactant (TBAR) levels were adjusted by saline incubations. Prostanoids in guinea pig SMC cultures were measured either by radioimmunoassay (RIA) or the isolation by high performance liquid chromatography (HPLC) of labeled prostanoids from SMC prelabeled with [14C]AA. Cell morphology and viability were studied by staining with Giemsa, nile red, and propidium iodide. VLDL and LDLBHT had little effect on prostanoid synthesis. Low-TBAR-LDLOXID enhanced total prostanoid levels and diminished the release of labeled prostanoids. Similar effects were found with exogenous free AA (unlabeled). Low-TBAR-LDLOXID did not affect the release of endogenous phospholipid AA as free AA. Synergism occurred between LDLOXID and exogenous free AA in prostanoid synthesis. Low-TBAR-LDLOXID evidently enhanced prostanoid levels in SMC both by supplying AA and by stimulating cyclooxygenase. High-TBAR-LDLOXID blocked prostanoid synthesis and enhanced cell death but time and pulse- recovery experiments showed that these effects were unrelated. High- TBAR-LDLOXID stimulated prostanoid synthesis when BHT was added to the incubation media. High-TBAR-LDLOXID also caused massive free AA release and the formation of many nonprostanoid derivatives. High-TBAR-LDLOXID evidently diminished overall prostanoid levels in SMC by inhibiting cyclooxygenase and at the same time stimulating AA release and the formation of other AA derivatives.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Vinals, J. Martinez-Gonzalez, and L. Badimon Regulatory Effects of HDL on Smooth Muscle Cell Prostacyclin Release Arterioscler. Thromb. Vasc. Biol., October 1, 1999; 19(10): 2405 - 2411. [Abstract] [Full Text] [PDF]

				M. Vinals, J. Martinez-Gonzalez, J. J. Badimon, and L. Badimon HDL-Induced Prostacyclin Release in Smooth Muscle Cells Is Dependent on Cyclooxygenase-2 (Cox-2) Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3481 - 3488. [Abstract] [Full Text]

				P. Vijayagopal and D. L. Glancy Macrophages Stimulate Cholesteryl Ester Accumulation in Cocultured Smooth Muscle Cells Incubated With Lipoprotein-Proteoglycan Complex Arterioscler. Thromb. Vasc. Biol., September 1, 1996; 16(9): 1112 - 1121. [Abstract] [Full Text]