Journal of Lipid Research, Vol 31, 653-658, Copyright © 1990 by Lipid Research, Inc.

ARTICLES

Sterol effects on phospholipid biosynthesis in the yeast strain GL7

M Ramgopal, M Zundel and K Bloch
James Bryant Conant Laboratories, Department of Chemistry, Harvard University, Cambridge, MA 02138.

Cells of the yeast sterol auxotroph GL7 were grown on either ergosterol or cholesterol to mid-logarithmic phase and total membrane fractions prepared. Activities of phospholipid biosynthetic enzymes in the two cell types were determined. The rates of phosphatidyl-ethanolamine- phosphatidyl-choline-N-methyl transferase and acyl-CoA-alpha-glycerol-3- phosphate transcylase were significantly greater in ergosterol-grown than in cholesterol-grown cells. These reactions were also inhibited by the polyene antibiotic filipin. By contrast the activities of long- chain fatty acyl-CoA synthetase, CTP-phosphatidate-cytidyl transferase, phosphatidylserine decarboxylase and of phosphatidylinositol synthetase were identical in the two (ergosterol and cholesterol) cultures and unaffected by filipin. The ergosterol effect on phosphatidyl- ethanolamine N-methyl transferase was greatest in cells harvested in early log phase, intermediate in the mid-log phase cells, and not significant in stationary phase cells.
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