J. Lipid Res. Acyl Labeled PIP's available August 1, 2008
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Journal of Lipid Research, Vol 31, 735-738, Copyright © 1990 by Lipid Research, Inc.

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Cholesterol 26-hydroxylase activity of hamster liver mitochondria: isotope ratio analysis using deuterated 26-hydroxycholesterol

E Kok and NB Javitt
Division of Hepatic Diseases, New York University Medical Center, NY 10016.

Deuterated 26-hydroxycholesterol prepared from diosgenin by modifications of existing methods permitted the determination of mitochondrial cholesterol 26-hydroxylase using endogenous cholesterol as the substrate. Enzyme activity in a group of Syrian hamsters was found to be 10.3 +/- 3.7 pmol.min-1.mg protein-1.
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