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Journal of Lipid Research, Vol 31, 1073-1081, Copyright © 1990 by Lipid Research, Inc.

ARTICLES

High-resolution proton nuclear magnetic resonance characterization of seminolipid from bovine spermatozoa

JG Alvarez, BT Storey, ML Hemling and RL Grob
Department of Physiology, University of Pennsylvania, School of Medicine, Philadelphia 19104.

The high-resolution one- and two-dimensional proton nuclear magnetic resonance (1H-NMR) characterization of seminolipid from bovine spermatozoa is presented. The 1H-NMR data was confirmed by gas-liquid chromatography-mass spectrometric analysis of the partially methylated alditol acetates of the sugar unit, mild alkaline methanolysis of the glyceryl ester, mobility on normal phase and diphasic thin-layer chromatography (HPTLC), and fast atom bombardment mass spectrometry (FAB-MS). The structure of the molecule corresponds to 1-O-hexadecyl-2- O-hexadecanoyl-3-O-beta-D-(3'-sulfo)-galactopyranosyl- sn-glycerol.
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