HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Satoh, O. Articles by Inoue, K. Search for Related Content PubMed PubMed Citation Articles by Satoh, O. Articles by Inoue, K. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 31, 1293-1300, Copyright © 1990 by Lipid Research, Inc.

ARTICLES

Lipid composition of hepatitis B virus surface antigen particles and the particle-producing human hepatoma cell lines

O Satoh, M Umeda, H Imai, H Tunoo and K Inoue
Mieji Institute of Health Science, Kanagawa, Japan.

More than 90% of lipids of hepatitis B virus surface antigen (HBsAg) particles produced by two human hepatoma cell lines (huGK-14 and PLC/PRF/5) were composed of phospholipids, with phosphatidylcholine being the dominant component, accounting for more than 80% of total membrane lipids. Analysis of subclass compositions of phospholipids of HBsAg particles and the host cell lines revealed that 1,2-diacyl glycerophosphocholine was preferentially incorporated into the membrane of the HBsAg particles, although both host cell lines contained extremely high concentrations (more than 60% of total phospholipids) of ether-linked phospholipids. Phospholipids of other hepatoma cell lines (HuH-7, Hep-G2, and huL-1) which were not associated with hepatitis B virus (HBV) infection, were composed mostly of 1,2- diacylglycerophospholipids. Activities of dihydroxyacetone-phosphate acyltransferase, which is known to be an obligatory enzyme in ether lipid biosynthesis, were found to be elevated by three- to fourfold in both huGK-14 and PLC/PRF/5 cells compared to those of other hepatoma cell lines. The results suggest a possible relationship between HBV- induced hepatocellular carcinogenesis and the drastic change in the metabolism of membrane phospholipids.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				U. Kato, K. Emoto, C. Fredriksson, H. Nakamura, A. Ohta, T. Kobayashi, K. Murakami-Murofushi, T. Kobayashi, and M. Umeda A Novel Membrane Protein, Ros3p, Is Required for Phospholipid Translocation across the Plasma Membrane in Saccharomyces cerevisiae J. Biol. Chem., September 27, 2002; 277(40): 37855 - 37862. [Abstract] [Full Text] [PDF]

				P. Vanlandschoot, F. Van Houtte, A. Roobrouck, A. Farhoudi, F. Stelter, D. L. Peterson, J. Gomez-Gutierrez, F. Gavilanes, and G. Leroux-Roels LPS-binding protein and CD14-dependent attachment of hepatitis B surface antigen to monocytes is determined by the phospholipid moiety of the particles J. Gen. Virol., September 1, 2002; 83(9): 2279 - 2289. [Abstract] [Full Text] [PDF]