HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vance, J. E. Articles by Russell, J. C. Search for Related Content PubMed PubMed Citation Articles by Vance, J. E. Articles by Russell, J. C. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 31, 1491-1501, Copyright © 1990 by Lipid Research, Inc.

ARTICLES

Hypersecretion of VLDL, but not HDL, by hepatocytes from the JCR:LA- corpulent rat

JE Vance and JC Russell
Lipid and Lipoprotein Group, University of Alberta, Edmonton, Canada.

The JCR:LA-corpulent male rat, when homozygous for the cp gene (cp/cp) is hyperlipidemic and prone to atherosclerosis. Both male and female cp/cp rats have markedly elevated serum levels of triacylglycerols and phospholipids [Dolphin, P.J. et al. 1987. Biochim. Biophys. Acta. 919: 140-148]. In the present study, monolayer cultures of hepatocytes were prepared from male and female, corpulent and lean, rats. There was a marked hypersecretion of all very low density lipoprotein (VLDL) lipid and apoprotein components from corpulent-derived cells. The increased secretion most likely accounts for the increased levels of VLDL lipids and apoproteins previously observed in serum. In contrast, there was no difference between the corpulent and lean hepatocytes in their secretion of high density lipoprotein (HDL) lipids and apoproteins. The difference in triacylglycerol secretion between the lean and corpulent cells was sustained even when the cells were cultured for 24, 48, and 72 h prior to the experiment, by which time the hormonal differences between the corpulent and lean animals would have been largely eliminated. The magnitude of the difference in triacyglycerol secretion did not diminish with increasing time in culture. The biochemical basis responsible for the hypersecretion of VLDL has not yet been established. However, preliminary results suggest that there is an inherent difference in glycerolipid metabolism in the two types of hepatocytes.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Wang, M. M. Jacome-Sosa, M. R. Ruth, S. D. Goruk, M. J. Reaney, D. R. Glimm, D. C. Wright, D. F. Vine, C. J. Field, and S. D. Proctor Trans-11 Vaccenic Acid Reduces Hepatic Lipogenesis and Chylomicron Secretion in JCR:LA-cp Rats J. Nutr., November 1, 2009; 139(11): 2049 - 2054. [Abstract] [Full Text] [PDF]

				Y. Wang, J. Lu, M. R. Ruth, S. D. Goruk, M. J. Reaney, D. R. Glimm, D. F. Vine, C. J. Field, and S. D. Proctor Trans-11 Vaccenic Acid Dietary Supplementation Induces Hypolipidemic Effects in JCR:LA-cp Rats J. Nutr., November 1, 2008; 138(11): 2117 - 2122. [Abstract] [Full Text] [PDF]

				J. C. Russell, S. D. Proctor, S. E. Kelly, and D. N. Brindley Pair feeding-mediated changes in metabolism: stress response and pathophysiology in insulin-resistant, atherosclerosis-prone JCR:LA-cp rats Am J Physiol Endocrinol Metab, June 1, 2008; 294(6): E1078 - E1087. [Abstract] [Full Text] [PDF]

				L. L. Atkinson, S. E. Kelly, J. C. Russell, J. Bar-Tana, and G. D. Lopaschuk MEDICA 16 Inhibits Hepatic Acetyl-CoA Carboxylase and Reduces Plasma Triacylglycerol Levels in Insulin-Resistant JCR: LA-cp Rats Diabetes, May 1, 2002; 51(5): 1548 - 1555. [Abstract] [Full Text] [PDF]

				M. B. Elam, H. G. Wilcox, L. M. Cagen, X. Deng, R. Raghow, P. Kumar, M. Heimberg, and J. C. Russell Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR:LA-cp rat J. Lipid Res., December 1, 2001; 42(12): 2039 - 2048. [Abstract] [Full Text] [PDF]

				P. Legrand, D. Catheline, M.-C. Fichot, and P. Lemarchal Inhibiting Delta 9-Desaturase Activity Impairs Triacylglycerol Secretion in Cultured Chicken Hepatocytes J. Nutr., February 1, 1997; 127(2): 249 - 256. [Abstract] [Full Text]

				J. C. Russell, R. M. Amy, S. E. Graham, P. J. Dolphin, G. O. Wood, and J. Bar-Tana Inhibition of Atherosclerosis and Myocardial Lesions in the JCR:LA-cp Rat by ß,ß'-Tetramethylhexadecanedioic Acid (MEDICA 16) Arterioscler Thromb Vasc Biol, July 1, 1995; 15(7): 918 - 923. [Abstract] [Full Text]

				M Douaire, B Belloir, J. Guillemot, J. Fraslin, P Langlois, and J Mallard Lipogenic enzyme and apoprotein messenger RNAs in long-term primary culture of chicken hepatocytes J. Cell Sci., January 3, 1993; 104(3): 713 - 718. [Abstract] [PDF]