Journal of Lipid Research, Vol 31, 1517-1522, Copyright © 1990 by Lipid Research, Inc.
Size exclusion chromatography for extraction of serum bile acids
R Nuber, H Maucher and EF Stange
Department of Internal Medicine II, University of Ulm, FRG.
A major problem in the measurement of serum bile acids is their
quantitative extraction from the high molecular protein matrix. In our
hands, the standard techniques of adsorption and reversed-phase
chromatography have yielded incomplete recovery for different bile acids
(33-93%) and poor reproducibility. In contrast, with the novel extraction
procedure of size exclusion chromatography, recovery was nearly
quantitative (75-104%) and reproducibility was satisfactory. The described
method allowed for a reliable determination of serum bile acids in healthy
subjects and patients with liver cirrhosis. We conclude that size exclusion
chromatography for serum bile acid extraction is more reliable than
alternative techniques, because the separation by size is independent of
solubility, charge, and polarity.
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