J. Lipid Res.
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Journal of Lipid Research, Vol 32, 157-164, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Lipid composition of lysosomal multilamellar bodies of male mouse urine

SK Gross, PF Daniel, JE Evans and RH McCluer
Department of Biochemistry, Eunice Kennedy Shriver Center, Waltham, MA 02254.

Previous studies from our laboratory have shown that male C57BL/6J mice excrete into the urine multilamellar lysosomal bodies that contain specific neutral glycosphingolipids. These mice excrete approximately 20-30% of their kidney glycolipids each day. The significance and function of this secretion of multilamellar lysosomal organelles is unknown. To characterize these excreted bodies further, we report here their neutral lipid and phospholipid composition. The bodies were collected by differential centrifugation, extracted with chloroform- methanol, and lipids were fractionated into neutral lipids, glycolipids, and phospholipids. The neutral lipids consisted primarily of cholesterol, dolichol, and ubiquinone. The phospholipid fraction consisted primarily of a single molecular species of phosphatidylcholine. This lipid which comprises more than 90% of the total phospholipids was found to contain 16:0 ether and C22:6 n-3 fatty acid as determined by gas-liquid chromatography-mass spectrometry. The glycosphingolipids as reported previously consisted primarily of galabiosylceramides and globotriaosylceramides. This membrane lipid composition is different from any previously reported cellular organelle.
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Copyright © 1991 by the American Society for Biochemistry and Molecular Biology.