HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cathcart, M. K. Articles by Chisolm, G. M. Search for Related Content PubMed PubMed Citation Articles by Cathcart, M. K. Articles by Chisolm, G. M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 32, 63-70, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Lipoxygenase-mediated transformation of human low density lipoprotein to an oxidized and cytotoxic complex

MK Cathcart, AK McNally and GM Chisolm
Department of Immunology, Cleveland Clinic Foundation, OH 44195.

We have been studying the mechanisms involved in the oxidative modification of low density lipoprotein (LDL) that lead to its transformation to a cytotoxic complex. Here we examine the direct effect-of soybean lipoxygenase (SLO), a 15-lipoxygenase, on normal human LDL. SLO oxidized LDL and rendered it cytotoxic; agents known to interfere with lipoxygenase activity inhibited this reaction. Enhancement of both the SLO-mediated LDL oxidation and the conversion of LDL to a cytotoxin was observed when either superoxide dismutase or copper (II) (3,5,-diisopropylsalicylic acid)2, both of which dismute superoxide anion, were included during the incubation of SLO with LDL. In contrast, catalase inhibited this reaction in the presence or absence of agents that dismute superoxide anion. Thus, purified lipoxygenase can mediate LDL modification and superoxide anion inhibits this reaction, Furthermore, H2O2 is essential for SLO-mediated LDL oxidation and conversion of LDL to a cytotoxin.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. P. Lillis, L. B. Van Duyn, J. E. Murphy-Ullrich, and D. K. Strickland LDL Receptor-Related Protein 1: Unique Tissue-Specific Functions Revealed by Selective Gene Knockout Studies Physiol Rev, July 1, 2008; 88(3): 887 - 918. [Abstract] [Full Text] [PDF]

				M. Penumetcha, N. Khan, and S. Parthasarathy Dietary oxidized fatty acids: an atherogenic risk? J. Lipid Res., September 1, 2000; 41(9): 1473 - 1480. [Abstract] [Full Text]

				A. Tokumura, T. Sumida, M. Toujima, K. Kogure, K. Fukuzawa, Y. Takahashi, and S. Yamamoto Structural identification of phosphatidylcholines having an oxidatively shortened linoleate residue generated through its oxygenation with soybean or rabbit reticulocyte lipoxygenase J. Lipid Res., June 1, 2000; 41(6): 953 - 962. [Abstract] [Full Text]

				G. M. Chisolm III, S. L. Hazen, P. L. Fox, and M. K. Cathcart The Oxidation of Lipoproteins by Monocytes-Macrophages. BIOCHEMICAL AND BIOLOGICAL MECHANISMS J. Biol. Chem., September 10, 1999; 274(37): 25959 - 25962. [Full Text] [PDF]

				C. Gniwotta, J. D. Morrow, L. J. Roberts II, and H. Kuhn Prostaglandin F2-Like Compounds, F2-Isoprostanes, Are Present in Increased Amounts in Human Atherosclerotic Lesions Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 3236 - 3241. [Abstract] [Full Text]

				V. A. Folcik, R. Aamir, and M. K. Cathcart Cytokine Modulation of LDL Oxidation by Activated Human Monocytes Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 1954 - 1961. [Abstract] [Full Text]

				A. D. Watson, N. Leitinger, M. Navab, K. F. Faull, S. Horkko, J. L. Witztum, W. Palinski, D. Schwenke, R. G. Salomon, W. Sha, et al. Structural Identification by Mass Spectrometry of Oxidized Phospholipids in Minimally Oxidized Low Density Lipoprotein That Induce Monocyte/Endothelial Interactions and Evidence for Their Presence in Vivo J. Biol. Chem., May 23, 1997; 272(21): 13597 - 13607. [Abstract] [Full Text] [PDF]

				Q. Li and M. K. Cathcart Selective Inhibition of Cytosolic Phospholipase A2 in Activated Human Monocytes. REGULATION OF SUPEROXIDE ANION PRODUCTION AND LOW DENSITY LIPOPROTEIN OXIDATION J. Biol. Chem., January 24, 1997; 272(4): 2404 - 2411. [Abstract] [Full Text] [PDF]

				S. Rangaswamy, M. S. Penn, G. M. Saidel, and G. M. Chisolm Exogenous Oxidized Low-Density Lipoprotein Injures and Alters the Barrier Function of Endothelium in Rats In Vivo Circ. Res., January 1, 1997; 80(1): 37 - 44. [Abstract] [Full Text]

				J. A. Cornicelli, K. Welch, B. Auerbach, S. J. Feinmark, and A. Daugherty Mouse Peritoneal Macrophages Contain Abundant {omega}-6 Lipoxygenase Activity That Is Independent of Interleukin-4 Arterioscler. Thromb. Vasc. Biol., December 1, 1996; 16(12): 1488 - 1494. [Abstract] [Full Text]

				P. Lesnik, C. Dentan, A. Vonica, M. Moreau, and M. J. Chapman Tissue Factor Pathway Inhibitor Activity Associated With LDL Is Inactivated by Cell- and Copper-Mediated Oxidation Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1121 - 1130. [Abstract] [Full Text]

				J. A. Berliner, M. Navab, A. M. Fogelman, J. S. Frank, L. L. Demer, P. A. Edwards, A. D. Watson, and A. J. Lusis Atherosclerosis: Basic Mechanisms : Oxidation, Inflammation, and Genetics Circulation, May 1, 1995; 91(9): 2488 - 2496. [Abstract] [Full Text]

				W. Xu, Y. Takahashi, T. Sakashita, T. Iwasaki, H. Hattori, and T. Yoshimoto Low Density Lipoprotein Receptor-related Protein Is Required for Macrophage-mediated Oxidation of Low Density Lipoprotein by 12/15-Lipoxygenase J. Biol. Chem., September 21, 2001; 276(39): 36454 - 36459. [Abstract] [Full Text] [PDF]