HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Prack, M. M. Articles by Gwynne, J. Search for Related Content PubMed PubMed Citation Articles by Prack, M. M. Articles by Gwynne, J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 32, 1611-1618, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Relationship between apolipoprotein E mRNA expression and tissue cholesterol content in rat adrenal gland

MM Prack, M Nicosia, DL Williams and J Gwynne
Department of Pharmacological Sciences, State University of New York, Stony Brook 11794.

Among extrahepatic tissues the adrenal gland has one of the highest concentrations of apoE mRNA and the highest rate of apoE synthesis. In the present investigation several previously described in vivo treatments were used to assess the relationship between apoE expression and cellular cholesterol in the rat adrenal gland. Treatment of rats with 4-aminopyrazolo[3,4-d]pyrimidine (4-APP) to lower serum cholesterol concentration and deplete adrenal gland cholesterol content decreased adrenal gland apoE mRNA concentration. These adrenal responses were blocked by dexamethasone (DEX) suggesting that the effect of 4-APP occurred indirectly via stimulation of the adrenal gland by endogenous adrenocorticotrophic (ACTH). Relative to control rats, DEX treatment increased both adrenal gland cholesterol content and apoE mRNA concentration. Concurrent ACTH and DEX administration reduced both adrenal gland cholesterol content and apoE mRNA concentration relative to DEX-treated rats. ACTH administration also rapidly decreased adrenal gland apoE mRNA concentration and cholesterol content in rats pretreated with DEX. In all the above experiments, adrenal gland cholesterol content and apoE mRNA concentration were positively correlated (r = 0.78, P = 0.0001). In contrast, aminoglutethimide treatment, which blocks adrenal gland steroidogenesis and greatly increases adrenal gland cholesterol content, was without effect on apoE mRNA concentration. ACTH administration to rats treated with DEX + aminoglutethimide resulted in decreased adrenal apoE mRNA despite greatly increased adrenal cholesterol content. This uncoupling of adrenal gland cholesterol content and apoE mRNA concentration suggests that apoE mRNA expression and cellular cholesterol are regulated independently by ACTH.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. E. Thorngate, P. A. Strockbine, S. K. Erickson, and D. L. Williams Altered adrenal gland cholesterol metabolism in the apoE-deficient mouse J. Lipid Res., November 1, 2002; 43(11): 1920 - 1926. [Abstract] [Full Text] [PDF]

				C. V. Zerbinatti, L. P. Mayer, R. G. Audet, and C. A. Dyer Apolipoprotein E Is a Putative Autocrine Regulator of the Rat Ovarian Theca Cell Compartment Biol Reprod, April 1, 2001; 64(4): 1080 - 1089. [Abstract] [Full Text]

				F. E. Thorngate, L. L. Rudel, R. L. Walzem, and D. L. Williams Low Levels of Extrahepatic Nonmacrophage ApoE Inhibit Atherosclerosis Without Correcting Hypercholesterolemia in ApoE-Deficient Mice Arterioscler. Thromb. Vasc. Biol., August 1, 2000; 20(8): 1939 - 1945. [Abstract] [Full Text] [PDF]

				J. Raber, S. F. Akana, S. Bhatnagar, M. F. Dallman, D. Wong, and L. Mucke Hypothalamic-Pituitary-Adrenal Dysfunction in Apoe-/- Mice: Possible Role in Behavioral and Metabolic Alterations J. Neurosci., March 1, 2000; 20(5): 2064 - 2071. [Abstract] [Full Text] [PDF]

				T. A. Holwell, S. C. Schweitzer, M. E. Reyland, and R. M. Evans Vimentin-dependent utilization of LDL-cholesterol in human adrenal tumor cells is not associated with the level of expression of apoE, sterol carrier protein-2, or caveolin J. Lipid Res., August 1, 1999; 40(8): 1440 - 1452. [Abstract] [Full Text]

				S. Swarnakar, M. E. Reyland, J. Deng, S. Azhar, and D. L. Williams Selective Uptake of Low Density Lipoprotein-Cholesteryl Ester Is Enhanced by Inducible Apolipoprotein E Expression in Cultured Mouse Adrenocortical Cells J. Biol. Chem., May 15, 1998; 273(20): 12140 - 12147. [Abstract] [Full Text] [PDF]