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Journal of Lipid Research, Vol 32, 1709-1712, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

An enzyme-coupled assay for acyl-CoA synthetase [published erratum appears in J Lipid Res 1992 Mar;33(3):446]

K Ichihara and Y Shibasaki
Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.

An enzyme-coupled, colorimetric method for the assay of acyl-CoA synthetase is described. Acyl-CoA formed from fatty acid and CoA by acyl-CoA synthetase was dehydrogenated by acyl-CoA oxidase. Hydrogen peroxide produced was then converted into formaldehyde in the presence of methanol by catalase. The formaldehyde reacted with a triazole compound, 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole, in an alkaline condition to form a purple dye, and the absorbance was measured spectrophotometrically.
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