Journal of Lipid Research, Vol 32, 1712-1715, Copyright © 1991 by Lipid Research, Inc.
Rapid isolation of monosialogangliosides from bovine brain gangliosides by selective-overload chromatography
O Koul, M Prada-Maluf, RH McCluer and MD Ullman
Division of Biochemistry, E. K. Shriver Center, Waltham, MA 02254.
A procedure for rapid isolation of monosialogangliosides from purified
bovine brain gangliosides has been developed. It utilizes the selective
difference in association between monosialogangliosides and
polysialogangliosides for the ion-exchange resin Q-Sepharose. When the
ion-exchange column is overloaded with a bovine brain ganglioside mixture
in the proper ganglioside to column bed-volume ratio, the
polysialogangliosides are selectively retained by the column while the
monosialogangliosides emerge with the void volume without the use of salt
for elution. With the critical ganglioside to bed-volume ratio (1 g:8.32
ml), and an appropriate column bed-height to column radius ratio of 6.9,
monosialogangliosides are reproducibly obtained in high purity with greater
than 90% yield. The method has been used at both the analytical and
preparative scale. We call this separation technique selective-overload
chromatography.
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