HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schneider, S. Articles by Kurz, G. Search for Related Content PubMed PubMed Citation Articles by Schneider, S. Articles by Kurz, G. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 32, 1755-1767, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Fluorescent derivatives of bile salts. I. Synthesis and properties of NBD-amino derivatives of bile salts

S Schneider, U Schramm, A Schreyer, HP Buscher, W Gerok and G Kurz
Institut fur Organische Chemie und Biochemie Universitat Freiburg, Germany.

In order to visualize bile salt transport, fluorescent bile salt derivatives were synthesized by introduction of the relatively small fluorescent 4-nitrobenzo-2-oxa-1,3-diazol (NBD)-amino group in either the 3-, 7-, or 12-position of the steroid structure, thus providing a complete set of diastereomeric derivatives, 3 alpha-NBD-amino-7 alpha,12 alpha-dihydroxy-5 beta-cholan-24-oic acid, 3 beta-NBD-amino-7 alpha,12 alpha-dihydroxy-5 beta-cholan-24-oic acid, 7 alpha-NBD-amino-3 alpha,12 alpha-dihydroxy-5 beta-cholan-24-oic acid, 7 beta-NBD-amino-3 alpha,12 alpha-dihydroxy-5 beta-cholan-24-oic acid, 12 alpha-NBD-amino- 3 alpha,7 alpha-dihydroxy-5 beta-cholan-24-oic acid, 12 beta-NBD-amino- 3 alpha,7 alpha-dihydroxy-5 beta-cholan-24-oic acid, as well as their taurine conjugates. Their optical properties with absorption maxima at about 490 nm and emission maxima at 550 nm make them suitable for fluorescent microscopic studies. Fluorescence of the NBD-derivatives is strongly dependent on polarity of the solvent, on the concentration of the bile salt derivatives, and only slightly on temperature.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Mennerick, M. Lamberta, H.-J. Shu, J. Hogins, C. Wang, D. F. Covey, L. N. Eisenman, and C. F. Zorumski Effects on Membrane Capacitance of Steroids with Antagonist Properties at GABAA Receptors Biophys. J., July 1, 2008; 95(1): 176 - 185. [Abstract] [Full Text] [PDF]

				J. F. Rebbeor, G. C. Connolly, J. H. Henson, J. L. Boyer, and N. Ballatori ATP-dependent GSH and glutathione S-conjugate transport in skate liver: role of an Mrp functional homologue Am J Physiol Gastrointest Liver Physiol, August 1, 2000; 279(2): G417 - G425. [Abstract] [Full Text] [PDF]

				N. Ballatori, J. F. Rebbeor, G. C. Connolly, D. J. Seward, B. E. Lenth, J. H. Henson, P. Sundaram, and J. L. Boyer Bile salt excretion in skate liver is mediated by a functional analog of Bsep/Spgp, the bile salt export pump Am J Physiol Gastrointest Liver Physiol, January 1, 2000; 278(1): G57 - G63. [Abstract] [Full Text] [PDF]

				W. Kramer, S. Stengelin, K.-H. Baringhaus, A. Enhsen, H. Heuer, W. Becker, D. Corsiero, F. Girbig, R. Noll, and C. Weyland Substrate specificity of the ileal and the hepatic Na+/bile acid cotransporters of the rabbit. I. Transport studies with membrane vesicles and cell lines expressing the cloned transporters J. Lipid Res., September 1, 1999; 40(9): 1604 - 1617. [Abstract] [Full Text]