HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rader, D. J. Articles by Brewer, H. B. Search for Related Content PubMed PubMed Citation Articles by Rader, D. J. Articles by Brewer, H. B., Jr Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 32, 1849-1859, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

In vivo metabolism of apolipoprotein A-I on high density lipoprotein particles LpA-I and LpA-I,A-II

DJ Rader, G Castro, LA Zech, JC Fruchart and HB Brewer Jr
Molecular Disease Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.

Apolipoprotein (apo) A-I is the major protein in high density lipoproteins (HDL) and is found in two major subclasses of lipoproteins, those containing apolipoprotein A-II (termed LpA-I,A-II) and those without apoA-II (termed LpA-I). The in vivo kinetics of apoA- I on LpA-I and LpA-I,A-II were investigated in normolipidemic human subjects. In the first series of studies, radiolabeled apoA-I and apoA- II were reassociated with autologous plasma lipoproteins and injected into normal subjects. LpA-I and LpA-I,A-II were isolated from plasma at selected time points by immunoaffinity chromatography. By 24 h after injection, only 52.8 +/- 1.0% of the apoA-I in LpA-I remained, whereas 66.9 +/- 2.7% of apoA-I in LpA-I,A-II remained (P less than 0.01). In the second series of studies, purified apoA-I was labeled with either 131I or 125I and reassociated with autologous plasma. Isolated LpA-I and LpA-I,A-II particles differentially labeled with 131I-labeled apoA- I and 125I-labeled apoA-I, respectively, were simultaneously injected into study subjects. The plasma residence time of apoA-I injected on LpA-I (mean 4.39 days) was substantially shorter than that of apoA-I injected on LpA-I,A-II (mean 5.17 days), with a mean difference in residence times of 0.79 +/- 0.08 days (P less than 0.001). These data demonstrate that apoA-I injected on LpA-I is catabolized more rapidly than apoA-I injected on LpA-I,A-II. The results are consistent with the concept that LpA-I and LpA-I,A-II have divergent metabolic pathways.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. M. M. Ooi, G. F. Watts, P. J. Nestel, D. Sviridov, A. Hoang, and P. H. R. Barrett Dose-Dependent Regulation of High-Density Lipoprotein Metabolism with Rosuvastatin in the Metabolic Syndrome J. Clin. Endocrinol. Metab., February 1, 2008; 93(2): 430 - 437. [Abstract] [Full Text] [PDF]

				S. Rashid, B. W. Patterson, and G. F. Lewis Thematic review series: Patient-Oriented Research. What have we learned about HDL metabolism from kinetics studies in humans? J. Lipid Res., August 1, 2006; 47(8): 1631 - 1642. [Abstract] [Full Text] [PDF]

				P. H. R. Barrett, D. C. Chan, and G. F. Watts Thematic review series: Patient-Oriented Research. Design and analysis of lipoprotein tracer kinetics studies in humans J. Lipid Res., August 1, 2006; 47(8): 1607 - 1619. [Abstract] [Full Text] [PDF]

				M. Nishiwaki, K. Ikewaki, G. Bader, H. Nazih, M. Hannuksela, A. T. Remaley, R. D. Shamburek, and H. B. Brewer Jr Human Lecithin:Cholesterol Acyltransferase Deficiency: In Vivo Kinetics of Low-Density Lipoprotein and Lipoprotein-X Arterioscler. Thromb. Vasc. Biol., June 1, 2006; 26(6): 1370 - 1375. [Abstract] [Full Text] [PDF]

				J. Ji, G. F. Watts, A. G. Johnson, D. C. Chan, E. M. M. Ooi, K.-A. Rye, A. P. Serone, and P. H. R. Barrett High-Density Lipoprotein (HDL) Transport in the Metabolic Syndrome: Application of a New Model for HDL Particle Kinetics J. Clin. Endocrinol. Metab., March 1, 2006; 91(3): 973 - 979. [Abstract] [Full Text] [PDF]

				N. J. Hime, K. J. Drew, K. Wee, P. J. Barter, and K.-A. Rye Formation of high density lipoproteins containing both apolipoprotein A-I and A-II in the rabbit J. Lipid Res., January 1, 2006; 47(1): 115 - 122. [Abstract] [Full Text] [PDF]

				M. C. de Beer, D. van der Westhuyzen, N. L. Whitaker, N. R. Webb, and F. C. de Beer SR-BI-mediated selective lipid uptake segregates apoA-I and apoA-II catabolism J. Lipid Res., October 1, 2005; 46(10): 2143 - 2150. [Abstract] [Full Text] [PDF]

				M. C. de Beer, L. W. Castellani, L. Cai, A. J. Stromberg, F. C. de Beer, and D. R. van der Westhuyzen ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36 J. Lipid Res., April 1, 2004; 45(4): 706 - 715. [Abstract] [Full Text] [PDF]

				K. Okubo, K. Ikewaki, S. Sakai, N. Tada, Y. Kawaguchi, and S. Mochizuki Abnormal HDL Apolipoprotein A-I and A-II Kinetics in Hemodialysis Patients: A Stable Isotope Study J. Am. Soc. Nephrol., April 1, 2004; 15(4): 1008 - 1015. [Abstract] [Full Text] [PDF]

				M. C. Cheung, S. D. Sibley, J. P. Palmer, J. F. Oram, and J. D. Brunzell Lipoprotein lipase and hepatic lipase: their relationship with HDL subspecies Lp(A-I) and Lp(A-I,A-II) J. Lipid Res., August 1, 2003; 44(8): 1552 - 1558. [Abstract] [Full Text] [PDF]

				M. T. Montoya, A. Porres, S. Serrano, J. C. Fruchart, P. Mata, J. A. G. Gerique, and G. R. Castro Fatty acid saturation of the diet and plasma lipid concentrations, lipoprotein particle concentrations, and cholesterol efflux capacity Am. J. Clinical Nutrition, March 1, 2002; 75(3): 484 - 491. [Abstract] [Full Text] [PDF]

				J. M. Martin-Campos, J. Julve, J. C. Escola, J. Ordonez-Llanos, J. Gomez, J. Binimelis, F. Gonzalez-Sastre, and F. Blanco-Vaca ApoA-IMALLORCA impairs LCAT activation and induces dominant familial hypoalphalipoproteinemia J. Lipid Res., January 1, 2002; 43(1): 115 - 123. [Abstract] [Full Text] [PDF]

				L.-C. Lyu, C.-Y. Yeh, A. H Lichtenstein, Z. Li, J. M Ordovas, and E. J Schaefer Association of sex, adiposity, and diet with HDL subclasses in middle-aged Chinese Am. J. Clinical Nutrition, July 1, 2001; 74(1): 64 - 71. [Abstract] [Full Text] [PDF]

				M. E. Brousseau, G. P. Eberhart, J. Dupuis, B. F. Asztalos, A. L. Goldkamp, E. J. Schaefer, and M. W. Freeman Cellular cholesterol efflux in heterozygotes for Tangier disease is markedly reduced and correlates with high density lipoprotein cholesterol concentration and particle size J. Lipid Res., July 1, 2000; 41(7): 1125 - 1135. [Abstract] [Full Text]

				U. J. F. Tietge, C. Maugeais, W. Cain, D. Grass, J. M. Glick, F. C. de Beer, and D. J. Rader Overexpression of Secretory Phospholipase A2 Causes Rapid Catabolism and Altered Tissue Uptake of High Density Lipoprotein Cholesteryl Ester and Apolipoprotein A-I J. Biol. Chem., March 31, 2000; 275(14): 10077 - 10084. [Abstract] [Full Text] [PDF]

				K. W. Huggins, E. R. Burleson, J. K. Sawyer, K. Kelly, L. L. Rudel, and J. S. Parks Determination of the tissue sites responsible for the catabolism of large high density lipoprotein in the African green monkey J. Lipid Res., March 1, 2000; 41(3): 384 - 394. [Abstract] [Full Text]

				W. Velez-Carrasco, A. H. Lichtenstein, Z. Li, G. G. Dolnikowski, S. Lamon-Fava, F. K. Welty, and E. J. Schaefer Apolipoprotein A-I and A-II Kinetic Parameters as Assessed by Endogenous Labeling With [2H3]Leucine in Middle-Aged and Elderly Men and Women Arterioscler. Thromb. Vasc. Biol., March 1, 2000; 20(3): 801 - 806. [Abstract] [Full Text] [PDF]

				M. N. Nanjee and E. A. Brinton Very Small Apolipoprotein A-I-containing Particles from Human Plasma: Isolation and Quantification by High-Performance Size-Exclusion Chromatography Clin. Chem., February 1, 2000; 46(2): 207 - 223. [Abstract] [Full Text] [PDF]

				P. L. Colvin, E. Moriguchi, P. H. R. Barrett, J. S. Parks, and L. L. Rudel Small HDL particles containing two apoA-I molecules are precursors in vivo to medium and large HDL particles containing three and four apoA-I molecules in nonhuman primates J. Lipid Res., October 1, 1999; 40(10): 1782 - 1792. [Abstract] [Full Text]

				S. M. Hammad, S. Stefansson, W. O. Twal, C. J. Drake, P. Fleming, A. Remaley, H. B. Brewer Jr., and W. S. Argraves Cubilin, the endocytic receptor for intrinsic factor-vitamin B12 complex, mediates high-density lipoprotein holoparticle endocytosis PNAS, August 31, 1999; 96(18): 10158 - 10163. [Abstract] [Full Text] [PDF]

				S. Braschi, T. A-M. Neville, M.-C. Vohl, and D. L. Sparks Apolipoprotein A-I charge and conformation regulate the clearance of reconstituted high density lipoprotein in vivo J. Lipid Res., March 1, 1999; 40(3): 522 - 532. [Abstract] [Full Text]

				P. Colvin, E. Moriguchi, H. Barrett, J. Parks, and L. Rudel Production rate determines plasma concentration of large high density lipoprotein in non-human primates J. Lipid Res., October 1, 1998; 39(10): 2076 - 2085. [Abstract] [Full Text]

				M. E. Brousseau, J. Wang, S. J. Demosky , Jr., B. L. Vaisman, G. D. Talley, S. Santamarina-Fojo, H. B. Brewer , Jr., and J. M. Hoeg Correction of hypoalphalipoproteinemia in LDL receptor-deficient rabbits by lecithin:cholesterol acyltransferase J. Lipid Res., August 1, 1998; 39(8): 1558 - 1567. [Abstract] [Full Text]

				B. Lamarche, K. D. Uffelman, G. Steiner, P. H. R. Barrett, and G. F. Lewis Analysis of particle size and lipid composition as determinants of the metabolic clearance of human high density lipoproteins in a rabbit model J. Lipid Res., June 1, 1998; 39(6): 1162 - 1172. [Abstract] [Full Text]

				M. Tilly-Kiesi, A. H. Lichtenstein, J. Joven, E. Vilella, M. C. Cheung, W. V. Carrasco, J. M. Ordovas, G. Dolnikowski, and E. J. Schaefer Impact of Gender on the Metabolism of Apolipoprotein A-I in HDL Subclasses LpAI and LpAI:AII in Older Subjects Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3513 - 3518. [Abstract] [Full Text]

				C. J. Packard and J. Shepherd Lipoprotein Heterogeneity and Apolipoprotein B Metabolism Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3542 - 3556. [Abstract] [Full Text]

				A. von Eckardstein, S. Kliesch, E. Nieschlag, A. Chirazi, G. Assmann, and H. M. Behre Suppression of Endogenous Testosterone in Young Men Increases Serum Levels of High Density Lipoprotein Subclass Lipoprotein A-I and Lipoprotein(a) J. Clin. Endocrinol. Metab., October 1, 1997; 82(10): 3367 - 3372. [Abstract] [Full Text] [PDF]

				M. Tilly-Kiesi, A. H. Lichtenstein, J. M. Ordovas, G. Dolnikowski, R. Malmstrom, M.-R. Taskinen, and E. J. Schaefer Subjects With ApoA-I(Lys107->0) Exhibit Enhanced Fractional Catabolic Rate of ApoA-I in Lp(AI) and ApoA-II in Lp(AI With AII) Arterioscler. Thromb. Vasc. Biol., May 1, 1997; 17(5): 873 - 880. [Abstract] [Full Text]

				R. McPherson, P. Lau, P. Kussie, H. Barrett, and A. R. Tall Plasma Kinetics of Cholesteryl Ester Transfer Protein in the Rabbit: Effects of Dietary Cholesterol Arterioscler. Thromb. Vasc. Biol., January 1, 1997; 17(1): 203 - 210. [Abstract] [Full Text]

				R. McPherson, G. Agnani, P. Lau, J.-C. Fruchart, A. D. Edgar, and Y. L. Marcel Role of Lp A-I and Lp A-I/A-II in Cholesteryl Ester Transfer Protein–Mediated Neutral Lipid Transfer: Studies in Normal Subjects and in Hypertriglyceridemic Patients Before and After Fenofibrate Therapy Arterioscler. Thromb. Vasc. Biol., November 1, 1996; 16(11): 1340 - 1346. [Abstract] [Full Text]

				E. A. Brinton Oral Estrogen Replacement Therapy in Postmenopausal Women Selectively Raises Levels and Production Rates of Lipoprotein A-I and Lowers Hepatic Lipase Activity Without Lowering the Fractional Catabolic Rate Arterioscler. Thromb. Vasc. Biol., March 1, 1996; 16(3): 431 - 440. [Abstract] [Full Text]

				E. Cavallero, F. Brites, B. Delfly, N. Nicolaiew, C. Decossin, C. De Geitere, J.-C. Fruchart, R. Wikinski, B. Jacotot, and G. Castro Abnormal Reverse Cholesterol Transport in Controlled Type II Diabetic Patients : Studies on Fasting and Postprandial LpA-I Particles Arterioscler. Thromb. Vasc. Biol., December 1, 1995; 15(12): 2130 - 2135. [Abstract] [Full Text]

				Y. Huang, A. von Eckardstein, S. Wu, and G. Assmann Cholesterol Efflux, Cholesterol Esterification, and Cholesteryl Ester Transfer by LpA-I and LpA-I/A-II in Native Plasma Arterioscler. Thromb. Vasc. Biol., September 1, 1995; 15(9): 1412 - 1418. [Abstract] [Full Text]

				M. Syvanne, J. Kahri, K. S. Virtanen, and M.-R. Taskinen HDLs Containing Apolipoproteins A-I and A-II (LpA-I:A-II) as Markers of Coronary Artery Disease in Men With Non–Insulin- Dependent Diabetes Mellitus Circulation, August 1, 1995; 92(3): 364 - 370. [Abstract] [Full Text]

				W. S. Davidson, W. V. Rodrigueza, S. Lund-Katz, W. J. Johnson, G. H. Rothblat, and M. C. Phillips Effects of Acceptor Particle Size on the Efflux of Cellular Free Cholesterol J. Biol. Chem., July 21, 1995; 270(29): 17106 - 17113. [Abstract] [Full Text] [PDF]

				L. Lagrost, C. Dengremont, A. Athias, C. d. Geitere, J.-C. Fruchart, C. Lallemant, P. Gambert, and G. Castro Modulation of Cholesterol Efflux from Fu5AH Hepatoma Cells by the Apolipoprotein Content of High Density Lipoprotein Particles J. Biol. Chem., June 2, 1995; 270(22): 13004 - 13009. [Abstract] [Full Text] [PDF]

				W. S. Davidson, K. L. Gillotte, S. Lund-Katz, W. J. Johnson, G. H. Rothblat, and M. C. Phillips The Effect of High Density Lipoprotein Phospholipid Acyl Chain Composition on the Efflux of Cellular Free Cholesterol J. Biol. Chem., March 17, 1995; 270(11): 5882 - 5890. [Abstract] [Full Text] [PDF]

				K. Ikewaki, L. A. Zech, M. Kindt, H. B. Brewer Jr, and D. J. Rader Apolipoprotein A-II Production Rate Is a Major Factor Regulating the Distribution of Apolipoprotein A-I Among HDL Subclasses LpA-I and LpA-I:A-II in Normolipidemic Humans Arterioscler. Thromb. Vasc. Biol., March 1, 1995; 15(3): 306 - 312. [Abstract] [Full Text]

				M. C. de Beer, D. M. Durbin, L. Cai, N. Mirocha, A. Jonas, N. R. Webb, F. C. de Beer, and D. R. van der Westhuyzen Apolipoprotein A-II Modulates the Binding and Selective Lipid Uptake of Reconstituted High Density Lipoprotein by Scavenger Receptor BI J. Biol. Chem., May 4, 2001; 276(19): 15832 - 15839. [Abstract] [Full Text] [PDF]