HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fong, L. G. Articles by Cooper, A. D. Search for Related Content PubMed PubMed Citation Articles by Fong, L. G. Articles by Cooper, A. D. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 32, 1899-1910, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Inhibition of lipopolysaccharide-induced interleukin-1 beta mRNA expression in mouse macrophages by oxidized low density lipoprotein

LG Fong, TA Fong and AD Cooper
Research Institute, Palo Alto Medical Foundation, CA 94301.

Recent studies have demonstrated the expression of messenger RNA (mRNA) for several cytokines within atherosclerotic arteries. Since cytokines have been shown to modulate functions of cultured arterial wall cells in a manner that could influence atherogenesis, this suggests that factors that modulate cytokine production would influence the atherosclerotic process. To examine whether lipoproteins can modulate cytokine production, the effect of lipoproteins on mouse macrophage interleukin-1 beta (IL-1 beta) mRNA expression was examined by dot blot and Northern blot analyses. Low density lipoprotein (LDL), acetylated- LDL, or malondialdehyde-LDL did not induce IL-1 beta mRNA expression or affect the expression in response to lipopolysaccharide (LPS). Similarly, copper ion-oxidized LDL did not stimulate the production of IL-1 beta mRNA. However, oxidized LDL inhibited the LPS-induced expression in a concentration- and time-dependent manner with a maximum inhibition (greater than 90%) observed after a 2.5 h preincubation with 25 micrograms protein/ml. These conditions did not affect protein synthesis or phagocytosis and the inhibition was partially reversible after 24 h, which together suggest that the inhibition was not due to cell death. An inhibition of IL-1 alpha and IL-6 mRNA expression was also observed while there was no change in gamma-actin mRNA levels. The level of inhibition of IL-1 beta mRNA was dependent upon the extent of LDL oxidation, but did not correlate with recognition by the scavenger receptor. A non-receptor pathway was supported by two lines of evidence: 1) the inhibition could be reproduced with a lipid extract, and 2) oxidized LDL also inhibited scavenger receptor negative THP-1 cell IL-1 beta mRNA expression. Finally, oxidized LDL had no effect on the turnover of IL-1 beta mRNA, suggesting that the decreased accumulation of IL-1 beta mRNA is due to a decrease in gene transcription. Together these studies suggest that as macrophages become foam cells their immune responsiveness is attenuated.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Qiu, A. C. Ho, W. Yu, and J. S. Hill Suppression of endothelial or lipoprotein lipase in THP-1 macrophages attenuates proinflammatory cytokine secretion J. Lipid Res., February 1, 2007; 48(2): 385 - 394. [Abstract] [Full Text] [PDF]

				L. Billiet, C. Furman, G. Larigauderie, C. Copin, K. Brand, J.-C. Fruchart, and M. Rouis Extracellular Human Thioredoxin-1 Inhibits Lipopolysaccharide-induced Interleukin-1{beta} Expression in Human Monocyte-derived Macrophages J. Biol. Chem., December 2, 2005; 280(48): 40310 - 40318. [Abstract] [Full Text] [PDF]

				S. Eligini, M. Brambilla, C. Banfi, M. Camera, L. Sironi, S. S. Barbieri, J. Auwerx, E. Tremoli, and S. Colli Oxidized phospholipids inhibit cyclooxygenase-2 in human macrophages via nuclear factor-{kappa}B/I{kappa}B- and ERK2-dependent mechanisms Cardiovasc Res, August 1, 2002; 55(2): 406 - 415. [Abstract] [Full Text] [PDF]

				M. S. Landis, H. V. Patel, and J. P. Capone Oxysterol Activators of Liver X Receptor and 9-cis-Retinoic Acid Promote Sequential Steps in the Synthesis and Secretion of Tumor Necrosis Factor-alpha from Human Monocytes J. Biol. Chem., February 8, 2002; 277(7): 4713 - 4721. [Abstract] [Full Text] [PDF]

				L. G. Fong and D. Le The Processing of Ligands by the Class A Scavenger Receptor Is Dependent on Signal Information Located in the Cytoplasmic Domain J. Biol. Chem., December 17, 1999; 274(51): 36808 - 36816. [Abstract] [Full Text] [PDF]

				S. Eligini, S. Colli, F. Basso, L. Sironi, and E. Tremoli Oxidized Low Density Lipoprotein Suppresses Expression of Inducible Cyclooxygenase in Human Macrophages Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1719 - 1725. [Abstract] [Full Text] [PDF]

				S. Page, C. Fischer, B. Baumgartner, M. Haas, U. Kreusel, G. Loidl, M. Hayn, H. W. L. Ziegler-Heitbrock, D. Neumeier, and K. Brand 4-Hydroxynonenal Prevents NF-{kappa}B Activation and Tumor Necrosis Factor Expression by Inhibiting I{kappa}B Phosphorylation and Subsequent Proteolysis J. Biol. Chem., April 23, 1999; 274(17): 11611 - 11618. [Abstract] [Full Text] [PDF]

				M. M. E. D. van den Eijnden, J. T. van Noort, L. Hollaar, A. van der Laarse, and R. M. Bertina Cholesterol or Triglyceride Loading of Human Monocyte-Derived Macrophages by Incubation With Modified Lipoproteins Does Not Induce Tissue Factor Expression Arterioscler. Thromb. Vasc. Biol., February 1, 1999; 19(2): 384 - 392. [Abstract] [Full Text] [PDF]

				Y. Fujioka, A. D. Cooper, and L. G. Fong Multiple processes are involved in the uptake of chylomicron remnants by mouse peritoneal macrophages J. Lipid Res., December 1, 1998; 39(12): 2339 - 2349. [Abstract] [Full Text]

				K. Brand, T. Eisele, U. Kreusel, M. Page, S. Page, M. Haas, A. Gerling, C. Kaltschmidt, F.-J. Neumann, N. Mackman, et al. Dysregulation of Monocytic Nuclear Factor-{kappa}B by Oxidized Low-Density Lipoprotein Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 1901 - 1909. [Abstract] [Full Text]

				S. Jovinge, M. P.S. Ares, B. Kallin, and J. Nilsson Human Monocytes/Macrophages Release TNF-{alpha} in Response to Ox-LDL Arterioscler. Thromb. Vasc. Biol., December 1, 1996; 16(12): 1573 - 1579. [Abstract] [Full Text]

				D. Shiffman, T. Mikita, J. T. N. Tai, D. P. Wade, J. G. Porter, J. J. Seilhamer, R. Somogyi, S. Liang, and R. M. Lawn Large Scale Gene Expression Analysis of Cholesterol-loaded Macrophages J. Biol. Chem., November 22, 2000; 275(48): 37324 - 37332. [Abstract] [Full Text] [PDF]