Journal of Lipid Research, Vol 32, 371-374, Copyright © 1991 by Lipid Research, Inc.
A simple method for the determination of lipid composition of human bile
M Muraca, MT Vilei, L Miconi, P Petrin, M Antoniutti and S Pedrazzoli
Istituto di Medicina Interna Cattedra di Patologia Chirurgica, University of Padua, Italy.
The Entero-Test, a device for easy sampling of gastrointestinal contents,
including bile, has been used for determination of biliary lipid
composition. The device consists of a weighted gelatin capsule containing
140 cm of a highly absorbent nylon line. The capsule is swallowed while one
end of the string is taped to the face. After 3.5 h, when the line has
reached the duodenum, gallbladder contraction is stimulated by
intramuscular administration of ceruletide. The line is pulled out, and the
last 15 cm are eluted four times in methanol. Total bile acids (by 3
alpha-hydroxysteroid-dehydrogenase assay), individual bile acids (by high
performance liquid chromatography), phospholipids (by assay of
lipid-soluble phosphorus), and cholesterol (by gas-liquid chromatography)
are determined in the eluate. Tests in vitro demonstrated no preferential
binding and a good recovery of biliary lipids from the thread. Similar
values of biliary cholesterol saturation were obtained by means of duodenal
intubation and of the Entero-Test in a series of 12 subjects (r = 0.952).
In 5 subjects, individual bile acids were also measured and were found to
be similar with both techniques (r = 0.948). When the test was repeated
over 3 days in a series of 7 subjects, biliary cholesterol saturation was
found to be remarkably reproducible (CV = 7.6%). Thus, the Entero-Test is a
convenient technique for the determination of biliary lipid composition,
which can be particularly useful in longitudinal studies.
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