Journal of Lipid Research, Vol 32, 477-484, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Effect of N-linked glycosylation on hepatic lipase activity

G Stahnke, RC Davis, MH Doolittle, H Wong, MC Schotz and H Will
Max-Planck-Institut fur Biochemie, Martinsried/Munich, Federal Republic of Germany.

Hepatic lipase (HL) is a secretory protein synthesized in hepatocytes and bound to liver endothelium. Previous studies have suggested that HL N-linked glycans are required for catalytic activity. To directly test this hypothesis, Xenopus laevis oocytes were used to express native rat HL or HL lacking one or both N-linked glycosylation sites. The expressed and secreted native HL had an apparent molecular mass of 53 kDa, consistent with purified rat liver HL. The mutant lacking both glycosylation sites, while poorly secreted, had an apparent molecular mass of 48 kDa, the same size observed for HL after enzymatic removal of N-linked oligosaccharides. Mutants lacking one of the two sites were intermediate in size and showed reduced secretion. Each of these expressed and secreted proteins had full catalytic activity that was inhibited by antisera to rat HL. Thus, N-linked glycosylation of rat HL, while important to lipase secretion, is not essential for the expression of lipase activity.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Skropeta, C. Settasatian, M. R. McMahon, K. Shearston, D. Caiazza, K. C. McGrath, W. Jin, D. J. Rader, P. J. Barter, and K.-A. Rye N-Glycosylation regulates endothelial lipase-mediated phospholipid hydrolysis in apoE- and apoA-I-containing high density lipoproteins J. Lipid Res., September 1, 2007; 48(9): 2047 - 2057. [Abstract] [Full Text] [PDF]

				R. J. Brown, G. C. Miller, N. Griffon, C. J. Long, and D. J. Rader Glycosylation of endothelial lipase at asparagine-116 reduces activity and the hydrolysis of native lipoproteins in vitro and in vivo J. Lipid Res., May 1, 2007; 48(5): 1132 - 1139. [Abstract] [Full Text] [PDF]

				G. C. Miller, C. J. Long, E. D. Bojilova, D. Marchadier, K. O. Badellino, N. Blanchard, I. V. Fuki, J. M. Glick, and D. J. Rader Role of N-linked glycosylation in the secretion and activity of endothelial lipase J. Lipid Res., November 1, 2004; 45(11): 2080 - 2087. [Abstract] [Full Text] [PDF]

				B. Perret, L. Mabile, L. Martinez, F. Terce, R. Barbaras, and X. Collet Hepatic lipase: structure/function relationship, synthesis, and regulation J. Lipid Res., August 1, 2002; 43(8): 1163 - 1169. [Abstract] [Full Text] [PDF]