Journal of Lipid Research, Vol 32, 763-773, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Oxidation-labile subfraction of human plasma low density lipoprotein isolated by ion-exchange chromatography

H Shimano, N Yamada, S Ishibashi, H Mokuno, N Mori, T Gotoda, K Harada, Y Akanuma, T Murase and Y Yazaki
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

We isolated subfractions of human plasma low density lipoprotein (LDL) using ion-exchange chromatography. Plasma LDL from normolipidemic subjects were applied to a DEAE Sepharose 6B column. After elution of the bulk of LDL at 150 mM NaCl (the major fraction), the residual LDL was eluted at 500 mM NaCl and designated as the minor fraction. The minor fraction, only less than 1% of total LDL, tended to be somewhat similar in certain properties to oxidized LDL, e.g., an increased negative charge, higher protein/cholesterol ratio, and a higher flotation density than native LDL. These results were consistent with data reported by Avogaro et al. (1988. Arteriosclerosis. 8: 79-87). However, assays of 125I-labeled LDL binding activity for LDL receptors equal to that of the major fraction. Incorporation of [14C]oleate into cholesteryl ester [acyl-CoA:cholesterol acyltransferase (ACAT) activity] in mouse peritoneal macrophages incubated with the minor fraction was only slightly greater than that with the major fraction. Incubation of the minor fraction with 0.5 microM Cu2+ caused a remarkable stimulation of ACAT activity, while stimulation by the major fraction required incubation with 5 microM Cu2+, suggesting that the minor fraction was relatively labile to oxidation. The minor but definite presence of a plasma LDL subfraction more negative and susceptible to oxidation implicates the possibility of its association with atherogenesis.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. W. Gaubatz, B. K. Gillard, J. B. Massey, R. C. Hoogeveen, M. Huang, E. E. Lloyd, J. L. Raya, C.-y. Yang, and H. J. Pownall Dynamics of dense electronegative low density lipoproteins and their preferential association with lipoprotein phospholipase A2 J. Lipid Res., February 1, 2007; 48(2): 348 - 357. [Abstract] [Full Text] [PDF]

				J. L. Sanchez-Quesada, S. Benitez, C. Otal, M. Franco, F. Blanco-Vaca, and J. Ordonez-Llanos Density distribution of electronegative LDL in normolipemic and hyperlipemic subjects J. Lipid Res., May 1, 2002; 43(5): 699 - 705. [Abstract] [Full Text] [PDF]

				J. L. Sanchez-Quesada, A. Perez, A. Caixas, M. Rigla, A. Payes, S. Benitez, and J. Ordonez-Llanos Effect of Glycemic Optimization on Electronegative Low-Density Lipoprotein in Diabetes: Relation to Nonenzymatic Glycosylation and Oxidative Modification J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3243 - 3249. [Abstract] [Full Text] [PDF]

				Conxita De Castellarnau, J. L. Sanchez-Quesada, S. Benitez, R. Rosa, L. Caveda, L. Vila, and J. Ordonez-Llanos Electronegative LDL From Normolipemic Subjects Induces IL-8 and Monocyte Chemotactic Protein Secretion by Human Endothelial Cells Arterioscler. Thromb. Vasc. Biol., October 1, 2000; 20(10): 2281 - 2287. [Abstract] [Full Text] [PDF]

				B. Vedie, X. Jeunemaitre, J. L. Megnien, I. Myara, H. Trebeden, A. Simon, and N. Moatti Charge Heterogeneity of LDL in Asymptomatic Hypercholesterolemic Men Is Related to Lipid Parameters and Variations in the ApoB and CIII Genes Arterioscler. Thromb. Vasc. Biol., November 1, 1998; 18(11): 1780 - 1789. [Abstract] [Full Text] [PDF]

				I. Maor, T. Hayek, R. Coleman, and M. Aviram Plasma LDL Oxidation Leads to Its Aggregation in the Atherosclerotic Apolipoprotein E-Deficient Mice Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2995 - 3005. [Abstract] [Full Text]

				K. Demuth, I. Myara, B. Chappey, B. Vedie, M. A. Pech-Amsellem, M. E. Haberland, and N. Moatti A Cytotoxic Electronegative LDL Subfraction Is Present in Human Plasma Arterioscler. Thromb. Vasc. Biol., June 1, 1996; 16(6): 773 - 783. [Abstract] [Full Text]

				A. Sevanian, J. Hwang, H. Hodis, G. Cazzolato, P. Avogaro, and G. Bittolo-Bon Contribution of an In Vivo Oxidized LDL to LDL Oxidation and Its Association With Dense LDL Subpopulations Arterioscler. Thromb. Vasc. Biol., June 1, 1996; 16(6): 784 - 793. [Abstract] [Full Text]