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Journal of Lipid Research, Vol 32, 1195-1204, Copyright © 1991 by Lipid Research, Inc.
Cellular retinoic acid-binding protein messenger RNA: levels in rat tissues and localization in rat testis
N Rajan, GL Kidd, DA Talmage, WS Blaner, A Suhara and DS Goodman
Department of Medicine, Columbia University, New York 10032.
Studies were conducted to explore the tissue- and cell-specific regulation
of cellular retinoic acid-binding protein (CRABP) expression in the rat.
Two studies were carried out. The first explored the regulation of CRABP
mRNA levels in selected rat tissues by dietary retinoid status, and the
relationship between CRABP mRNA and protein levels in different tissues.
The second examined the cellular localization of CRABP expression in the
testis. In order to conduct these experiments, a cDNA encoding CRABP was
isolated and characterized. The DNA sequence of the coding region had 96%
identity with that of the mouse CRABP cDNA and encodes a protein identical
to mouse and bovine CRABP. CRABP mRNA and protein levels were quantified in
five tissues from normal, retinoid-deficient, and retinol-repleted rats.
Tissue CRABP and CRABP mRNA levels were highly correlated (P less than
0.01) indicating that inter-tissue variability of CRABP levels mainly
results from regulation of CRABP mRNA levels. Neither CRABP protein nor
mRNA levels were affected by retinol deficiency, in marked contrast with
results previously demonstrated with cellular retinol- binding protein
(CRBP) (J. Lipid Res. 1990. 31: 821-829). 35S-labeled CRABP cRNA probes
were used to localize CRABP mRNA within the testis of adult rats by in situ
hybridization. CRABP mRNA was localized selectively in the periphery of the
seminiferous tubules, primarily in type A spermatogonia. The localization
of CRABP mRNA differs from that of CRABP protein, which is known to be
enriched in maturing and more mature germinal cells. This difference
suggests that CRABP in germ cells may be highly stable, remaining in the
maturing germ cells without degradation long after CRABP mRNA levels have
declined to very low levels. The specific localization of CRABP mRNA and
protein presumably reflects the biological roles of retinoic acid in the
development and/or later function of germinal cells.

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Copyright © 1991 by the American Society for Biochemistry and Molecular Biology.
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