|
|
||||||||
Journal of Lipid Research, Vol 32, 1275-1280, Copyright © 1991 by Lipid Research, Inc.
ARTICLES |
Y Takada, J Sasaki, M Seki, S Ogata, Y Teranishi and K Arakawa
Department of Internal Medicine, University of Fukuoka School of Medicine, Japan.
A new genetic variant apolipoprotein (apo), A-I Yame, was discovered during screening for apoA-I genetic variants with isoelectric focusing gel electrophoresis. To investigate the structural abnormality of apoA- I Yame, we amplified the apoA-I gene isolated from the proband's peripheral blood leukocytes with the polymerase chain reaction (PCR) method and directly sequenced these PCR fragments. ApoA-I Yame was found to have aspartic acid (GAC) substituted by tyrosine (TAC) at residue 13. We also identified this substitution by an automated DNA sequencer. This substitution was confirmed with amino acid sequencing of the isolated apoA-I Yame by Immobiline gel electrophoresis. This combined method, direct PCR from genomic DNA-derived individual peripheral blood leukocytes and subsequent direct sequencing, can be used to identify the entire sequence of apoA-I in a short period of time. Furthermore, with this method, it is possible to identify both alleles in heterozygous individuals.
This article has been cited by other articles:
![]() |
P. G. Frank and Y. L. Marcel Apolipoprotein A-I: structure;-function relationships J. Lipid Res., June 1, 2000; 41(6): 853 - 872. [Abstract] [Full Text] |
||||
![]() |
H. Han, J. Sasaki, A. Matsunaga, H. Hakamata, W. Huang, M. Ageta, T. Taguchi, T. Koga, M. Kugi, S. Horiuchi, et al. A Novel Mutant, ApoA-I Nichinan (Glu235->0), Is Associated With Low HDL Cholesterol Levels and Decreased Cholesterol Efflux From Cells Arterioscler. Thromb. Vasc. Biol., June 1, 1999; 19(6): 1447 - 1455. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Matsunaga, J. Sasaki, H. Han, W. Huang, M. Kugi, T. Koga, S. Ichiki, T. Shinkawa, and K. Arakawa Compound Heterozygosity for an Apolipoprotein A1 Gene Promoter Mutation and a Structural Nonsense Mutation With Apolipoprotein A1 Deficiency Arterioscler. Thromb. Vasc. Biol., February 1, 1999; 19(2): 348 - 355. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Moriyama, J. Sasaki, Y. Takada, A. Matsunaga, J. Fukui, J. J. Albers, and K. Arakawa A Cysteine-Containing Truncated Apo A-I Variant Associated With HDL Deficiency Arterioscler. Thromb. Vasc. Biol., December 1, 1996; 16(12): 1416 - 1423. [Abstract] [Full Text] |
||||
![]() |
M. Tilly-Kiesi, Z. Qiuping, S. Ehnholm, J. Kahri, S. Lahdenpera, C. Ehnholm, and M.-R. Taskinen ApoA-IHelsinki (Lys107->0) Associated With Reduced HDL Cholesterol and LpA-I:A-II Deficiency Arterioscler. Thromb. Vasc. Biol., September 1, 1995; 15(9): 1294 - 1306. [Abstract] [Full Text] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |