HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sokol, R. J. Articles by Khandwala, R. A. Search for Related Content PubMed PubMed Citation Articles by Sokol, R. J. Articles by Khandwala, R. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 32, 1349-1357, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Effect of dietary lipid and vitamin E on mitochondrial lipid peroxidation and hepatic injury in the bile duct-ligated rat

RJ Sokol, M Devereaux and RA Khandwala
Department of Pediatrics, University of Colorado School of Medicine, Denver.

To assess whether lipid peroxidation of hepatic mitochondria is associated with cholestatic hepatic injury we examined the effect of bile duct ligation (BDL) versus sham surgery on mitochondrial lipids of rats maintained on one of seven diets. Diets included vitamin E- deficient (E-) and vitamin E-sufficient (E+) combined with normal lipid (11.9% calories as stripped corn oil), high lipid (35% calories as stripped corn oil), or n-3 fatty acid (fish oil) supplementation. Rats were killed 17 days after surgery, mitochondria were isolated by differential centrifugation, and lipid-conjugated dienes and thiobarbituric acid-reacting substances (TBARS) were measured in mitochondrial lipids as indices of lipid peroxidation. BDL resulted in significant increases in lipid peroxidation in all dietary groups. The E- high lipid diets (with either corn oil or fish oil) were associated with higher lipid peroxide and serum bilirubin values in BDL rats compared to the normal lipid diets. Fish oil supplementation did not ameliorate cholestatic or oxidative injury. Serum alanine aminotransferase, bilirubin, alkaline phosphatase, and cholylglycine levels correlated significantly with levels of mitochondrial conjugated dienes and TBARS. These data suggest that free radical stress occurs during BDL in the rat and may result in mitochondrial lipid peroxidation, and that diets high in lipid may increase free radical damage to hepatic mitochondria. The role of free radicals in cholestatic hepatic injury requires further investigation.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Z. Zhong, M. Froh, M. Lehnert, R. Schoonhoven, L. Yang, H. Lind, J. J. Lemasters, and R. G. Thurman Polyphenols from Camellia sinenesis attenuate experimental cholestasis-induced liver fibrosis in rats Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G1004 - G1013. [Abstract] [Full Text] [PDF]

				D Kumar and R K Tandon Fatigue in cholestatic liver disease--a perplexing symptom Postgrad. Med. J., July 1, 2002; 78(921): 404 - 407. [Abstract] [Full Text] [PDF]

				P Ljubuncic, Z Tanne, and A Bomzon Evidence of a systemic phenomenon for oxidative stress in cholestatic liver disease Gut, November 1, 2000; 47(5): 710 - 716. [Abstract] [Full Text] [PDF]

				G. A. Ramm, V. G. Nair, K. R. Bridle, R. W. Shepherd, and D. H. G. Crawford Contribution of Hepatic Parenchymal and Nonparenchymal Cells to Hepatic Fibrogenesis in Biliary Atresia Am. J. Pathol., August 1, 1998; 153(2): 527 - 535. [Abstract] [Full Text] [PDF]

				K. Chijiiwa, N. Kameoka, S. Saeki, M. Komura, K. Yamaguchi, S. Kuroki, and M. Tanaka Functional Contribution of Preoperative Portal Vein Occlusion to Hepatectomy: With Special Reference to Hepatic Energy Charge and DNA Synthesis After Hepatectomy in Rats Arch Surg, July 1, 1996; 131(7): 779 - 784. [Abstract] [PDF]