HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Amri, E. Z. Articles by Grimaldi, P. Search for Related Content PubMed PubMed Citation Articles by Amri, E. Z. Articles by Grimaldi, P. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 32, 1457-1463, Copyright © 1991 by Lipid Research, Inc.

ARTICLES

Regulation of adipose cell differentiation. II. Kinetics of induction of the aP2 gene by fatty acids and modulation by dexamethasone

EZ Amri, G Ailhaud and P Grimaldi
Centre de Biochimie (CNRS-UMR 134), U.F.R. Sciences, Universite de Nice- Sophia Antipolis, France.

Fatty acids behave as activators of the aP2 gene expression in committed, lipid-free, non-terminally differentiated Ob1771 cells. Like fatty acids, dexamethasone provokes a dose-dependent accumulation of aP2 mRNA. However, fatty acids and dexamethasone act through different mechanisms to activate the aP2 gene expression since i) fatty acids and dexamethasone act in a synergistic manner; ii) the effect of dexamethasone is rapid and transient (maximal effect after 8 h), whereas that of fatty acids is slower, and maintained as long as the inducer is present and is fully reversible upon fatty acid removal; iii) the induction of the aP2 gene expression by dexamethasone does not require ongoing protein synthesis, while the response to fatty acids is completely prevented by cycloheximide; and iv) the induction of the aP2 gene expression by fatty acids but not by dexamethasone is confined to preadipocyte cell lines. This suggests that the process of activation by fatty acids, rather than the expression of the aP2 gene, is unique to adipose cells. Besides their effects on the aP2 gene, fatty acids activate the expression of the acyl CoA synthetase gene which encodes another protein involved in fatty acid metabolism. Activation of both genes by fatty acids appears not to be mediated by the CCAAT enhancer binding protein, a nuclear factor reported as transactivator of the aP2 promoter activity, since the enhancer binding protein mRNA is not expressed under these conditions.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Sekiya, J.-i. Osuga, H. Okazaki, N. Yahagi, K. Harada, W.-J. Shen, Y. Tamura, S. Tomita, Y. Iizuka, K. Ohashi, et al. Absence of Hormone-sensitive Lipase Inhibits Obesity and Adipogenesis in Lepob/ob Mice J. Biol. Chem., April 9, 2004; 279(15): 15084 - 15090. [Abstract] [Full Text] [PDF]

				L. Sun, A. C. Nicholson, D. P. Hajjar, A. M. Gotto Jr., and J. Han Adipogenic differentiating agents regulate expression of fatty acid binding protein and CD36 in the J744 macrophage cell line J. Lipid Res., October 1, 2003; 44(10): 1877 - 1886. [Abstract] [Full Text] [PDF]

				M. Chen, Y. Yang, E. Braunstein, K. E. Georgeson, and C. M. Harmon Gut expression and regulation of FAT/CD36: possible role in fatty acid transport in rat enterocytes Am J Physiol Endocrinol Metab, November 1, 2001; 281(5): E916 - E923. [Abstract] [Full Text] [PDF]

				C. Bastie, D. Holst, D. Gaillard, C. Jehl-Pietri, and P. A. Grimaldi Expression of Peroxisome Proliferator-activated Receptor PPARdelta Promotes Induction of PPARgamma and Adipocyte Differentiation in 3T3C2 Fibroblasts J. Biol. Chem., July 30, 1999; 274(31): 21920 - 21925. [Abstract] [Full Text] [PDF]

				D. L. Crandall, D. C. Armellino, D. E. Busler, B. McHendry-Rinde, and J. G. Kral Angiotensin II Receptors in Human Preadipocytes: Role in Cell Cycle Regulation Endocrinology, January 1, 1999; 140(1): 154 - 158. [Abstract] [Full Text]

				N. Abumrad, C. Harmon, and A. Ibrahimi Membrane transport of long-chain fatty acids: evidence for a facilitated process J. Lipid Res., December 1, 1998; 39(12): 2309 - 2318. [Abstract] [Full Text]

				R. A. Baillie, X. Sha, P. Thuillier, and S. D. Clarke A novel 3T3-L1 preadipocyte variant that expresses PPAR{gamma}2 and RXR{alpha} but does not undergo differentiation J. Lipid Res., October 1, 1998; 39(10): 2048 - 2053. [Abstract] [Full Text]

				J.-J. Lee, P. J. Smith, and S. K. Fried Mechanisms of Decreased Lipoprotein Lipase Activity in Adipocytes of Starved Rats Depend on Duration of Starvation J. Nutr., June 1, 1998; 128(6): 940 - 946. [Abstract] [Full Text]

				M. Okuno, K. Kajiwara, S. Imai, T. Kobayashi, N. Honma, T. Maki, K. Suruga, T. Goda, S. Takase, Y. Muto, et al. Perilla Oil Prevents the Excessive Growth of Visceral Adipose Tissue in Rats by Down-Regulating Adipocyte Differentiation J. Nutr., September 1, 1997; 127(9): 1752 - 1757. [Abstract] [Full Text]

				L. Teboul, D. Gaillard, L. Staccini, H. Inadera, E.-Z. Amri, and P. A. Grimaldi Thiazolidinediones and Fatty Acids Convert Myogenic Cells into Adipose-like Cells J. Biol. Chem., November 24, 1995; 270(47): 28183 - 28187. [Abstract] [Full Text] [PDF]

				E.-Z. Amri, F.édér. Bonino, Gér. Ailhaud, N. A. Abumrad, and P. A. Grimaldi Cloning of a Protein That Mediates Transcriptional Effects of Fatty Acids in Preadipocytes J. Biol. Chem., February 3, 1995; 270(5): 2367 - 2371. [Abstract] [Full Text] [PDF]

				E. Duplus, M. Glorian, and C. Forest Fatty Acid Regulation of Gene Transcription J. Biol. Chem., September 29, 2000; 275(40): 30749 - 30752. [Full Text] [PDF]