Journal of Lipid Research, Vol 33, 89-95, Copyright © 1992 by Lipid Research, Inc.

ARTICLES

Effect of improved diabetes control on the expression of lipoprotein lipase in human adipose tissue

RB Simsolo, JM Ong, B Saffari and PA Kern
Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048.

Patients with diabetes commonly manifest hypertriglyceridemia along with decreased adipose tissue lipoprotein lipase (LPL) activity, and improved diabetes control tends to reverse these abnormalities. To better understand the mechanism of regulation of LPL in diabetes, 11 diabetic patients (3 type I, 8 type II) were brought under improved glycemic control, and adipose tissue LPL gene expression was assessed by performing paired fat biopsies. Six of the 11 patients attained improved control with insulin, with a decrease in glycohemoglobin (glyc Hgb) from 13.8 +/- 0.9 to 10.4 +/- 0.6%; 5 patients attained improved control with glyburide (glyc Hgb fell from 14.2 +/- 2.4 to 8.8 +/- 0.6%), and together they demonstrated a lowering of serum triglycerides and total cholesterol. No changes were observed in HDL cholesterol. Improved diabetes control resulted in a significant increase in LPL activity in both the heparin-releasable (HR) and extractable (EXT) fractions of adipose tissue, as well as in LPL immunoreactive mass. The change in LPL activity with improved control was variable, and showed a positive correlation with the HDL levels prior to treatment (r = 0.74, P less than 0.02). When adipose tissue was pulse-labeled with [35S]methionine, there was an increase in isotope incorporation into LPL after treatment, indicating an increase in LPL synthetic rate. However, improved diabetes control resulted in no significant change in LPL mRNA levels. Thus, improved glycemic control resulted in an increase in LPL activity which correlated with each patient's basal high density lipoprotein. This increase in LPL activity was accompanied by an increase in LPL immunoreactive mass, and an increase in LPL synthesis.(ABSTRACT TRUNCATED AT 250 WORDS)
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. A. Wagner, P. J. Larson, S. Weiss, J. L. Miller, T. W. Doebber, M. S. Wu, D. E. Moller, and K. M. Gottesdiener Individual and Combined Effects of Peroxisome Proliferator-Activated Receptor and {gamma} Agonists, Fenofibrate and Rosiglitazone, on Biomarkers of Lipid and Glucose Metabolism in Healthy Nondiabetic Volunteers J. Clin. Pharmacol., May 1, 2005; 45(5): 504 - 513. [Abstract] [Full Text] [PDF]

				P. A. Kern, G. Di Gregorio, T. Lu, N. Rassouli, and G. Ranganathan Perilipin Expression in Human Adipose Tissue Is Elevated with Obesity J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1352 - 1358. [Abstract] [Full Text] [PDF]

				L. L. Hensley, G. Ranganathan, E. M. Wagner, B. D. Wells, J. C. Daniel, D. Vu, C. F. Semenkovich, R. Zechner, and P. A. Kern Transgenic Mice Expressing Lipoprotein Lipase in Adipose Tissue: ABSENCE OF THE PROXIMAL 3'-UNTRANSLATED REGION CAUSES TRANSLATIONAL UPREGULATION J. Biol. Chem., August 29, 2003; 278(35): 32702 - 32709. [Abstract] [Full Text] [PDF]

				G. Ranganathan, D. Phan, I. D. Pokrovskaya, J. E. McEwen, C. Li, and P. A. Kern The Translational Regulation of Lipoprotein Lipase by Epinephrine Involves an RNA Binding Complex Including the Catalytic Subunit of Protein Kinase A J. Biol. Chem., November 1, 2002; 277(45): 43281 - 43287. [Abstract] [Full Text] [PDF]

				L. D. M. C.-B. Ferreira, P. U. Huey, B. E. Pulford, D. N. Ishii, and R. H. Eckel Sciatic Nerve Lipoprotein Lipase Is Reduced in Streptozotocin-Induced Diabetes and Corrected by Insulin Endocrinology, April 1, 2002; 143(4): 1213 - 1217. [Abstract] [Full Text] [PDF]

				M. Bergo, G. Wu, T. Ruge, and T. Olivecrona Down-regulation of Adipose Tissue Lipoprotein Lipase during Fasting Requires That a Gene, Separate from the Lipase Gene, Is Switched On J. Biol. Chem., March 29, 2002; 277(14): 11927 - 11932. [Abstract] [Full Text] [PDF]

				G. Ranganathan, R. Kaakaji, and P. A. Kern Role of Protein Kinase C in the Translational Regulation of Lipoprotein Lipase in Adipocytes J. Biol. Chem., March 26, 1999; 274(13): 9122 - 9127. [Abstract] [Full Text] [PDF]

				S. Ranganathan and P. A. Kern Thiazolidinediones Inhibit Lipoprotein Lipase Activity in Adipocytes J. Biol. Chem., October 2, 1998; 273(40): 26117 - 26122. [Abstract] [Full Text] [PDF]

				P. A. Kern Potential Role of TNFalpha and Lipoprotein Lipase as Candidate Genes for Obesity J. Nutr., September 1, 1997; 127(9): 1917 - 1917. [Abstract] [Full Text]

				S. Pillarisetti, L. Paka, A. Sasaki, T. Vanni-Reyes, B. Yin, N. Parthasarathy, W. D. Wagner, and I. J. Goldberg Endothelial Cell Heparanase Modulation of Lipoprotein Lipase Activity. EVIDENCE THAT HEPARAN SULFATE OLIGOSACCHARIDE IS AN EXTRACELLULAR CHAPERONE J. Biol. Chem., June 20, 1997; 272(25): 15753 - 15759. [Abstract] [Full Text] [PDF]

				G. Ranganathan, D. Vu, and P. A. Kern Translational Regulation of Lipoprotein Lipase by Epinephrine Involves a Trans-acting Binding Protein Interacting with the 3' Untranslated Region J. Biol. Chem., January 24, 1997; 272(4): 2515 - 2519. [Abstract] [Full Text] [PDF]

				X.L. Wang, R.M. McCredie, and D.E.L. Wilcken Common DNA Polymorphisms at the Lipoprotein Lipase Gene : Association With Severity of Coronary Artery Disease and Diabetes Circulation, April 1, 1996; 93(7): 1339 - 1345. [Abstract] [Full Text]

				G. Ranganathan, A. Yukht, M. Saghizadeh, R. B. Simsolo, A. Pauer, and P. A. Kern Tissue-specific Expression of Human Lipoprotein Lipase J. Biol. Chem., March 31, 1995; 270(13): 7149 - 7155. [Abstract] [Full Text] [PDF]

				G. Ranganathan, C. Li, and P. A. Kern The Translational Regulation of Lipoprotein Lipase in Diabetic Rats Involves the 3'-Untranslated Region of the Lipoprotein Lipase mRNA J. Biol. Chem., December 22, 2000; 275(52): 40986 - 40991. [Abstract] [Full Text] [PDF]

				P. M. Catalano, S. E. Nizielski, J. Shao, L. Preston, L. Qiao, and J. E. Friedman Downregulated IRS-1 and PPARgamma in obese women with gestational diabetes: relationship to FFA during pregnancy Am J Physiol Endocrinol Metab, March 1, 2002; 282(3): E522 - E533. [Abstract] [Full Text] [PDF]